Abstract
Summary
The pubic joint of the mouse was used as a model for the study of hormonal influences on acid phosphatase activity in connective tissue. Biochemically determined acid phosphatase was elevated in pubic joints of ovariectomized mice treated with an estrogen (ECP) and further increased by combined treatment with ECP and relaxin (R). The enhancement of enzyme activity was accompanied by transformation of the connective tissue from a cartilagenous to a ligamentous type. Progesterone (P) alone did not influence pubic joint acid phosphatase or modify the response to ECP. P appeared to reduce the expected increase in enzymatic activity in mice treated with ECP plus R and at the same time inhibited the transformation of pubic joint cartilage to ligament. Pubic joint acid phosphatase was very low in hypophysectomized mice. The enzymatic responses to ECP and ECP plus R observed in ovariectomized mice were not obtained in hypophysectomized mice unless STH was also administered. Again the changes in acid phosphatase were correlated with transformation of cartilage to ligament. The results are discussed in relation to a possible enzymatic basis for the phenomenon of pubic symphyseal relaxation.
We appreciate the skilled assistance of Margaret Butler, Sara Priester and Shirley Carter. The help and advice of Vivian Beach is gratefully acknowledged.
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