Induction of Neoplasia in vitro in Hamster Kidney Tissue by Adenovirus 7-SV40 “Hybrid” Strain (LLE46)

Abstract

Summary

Newborn hamster kidney cell cultures were transformed in vitro by LLE46 virus, an adenovirus 7-SV40 hybrid, and by SV40. The cultures transformed by both viruses consisted of rapidly growing cuboidal and polygonal cells, although those in the LLE46 cultures were smaller and had less tendency to grow in clumps. Neither infectious adenovirus 7 nor SV40 could be recovered from the transformed cells. More than 90% of the cells in both the LLE46 and the SV40 cultures contained persistent intranuclear SV40 T antigen while no adenovirus 12 or 7 T antigens were demonstrable. Tumors were rapidly produced when both types of transformed cells were injected into newborn hamsters, and into irradiated young adult hamsters. As previously described, the tumors produced by the SV40 transformed cells were predominantly carcinomas with areas of tubular differentiation. Those produced by the LLE46 transformed cells, however, were predominantly undifferentiated and histologically similar to adenovirus 12 tumors. Although they did contain a few small foci resembling SV40 sarcomas, no tubular epithelial structures were seen.