Abstract
Summary
Different optimal doses of heat-killed Mycobacterium bovis (BCG) were required to sensitize guinea pigs maximally toward fractions FB (largely protein) and GB (50% protein and 50% carbohydrate) prepared from culture filtrates of BCG. Increasing the sensitizing dose beyond the optimum resulted in lesser skin sensitivity for each antigen. No circulating antibodies were detected towards GB, which was the more skin reactive fraction. Complement-fixing and precipitating antibodies were produced toward FB but could not be correlated with the dose of injected organisms.
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