Abstract
Summary
5-Fluorodeoxyuridine (FUDR) was only partially effective in suppressing synthesis of canine hepatitis virus in prolonged cultures of dog kidney cells. Suppression was more effective when cultures were inoculated with large amounts of virus than with small amounts of virus. Examinations of coverslip cultures by acridine orange, fluorescent antibody, and by microspectrophoto-metry of material stained by the Feulgen method revealed DNA and virus in a few cells in each FUDR-inhibited culture. Usually there was no increase in the mean level of DNA per inhibited culture. It was concluded that the multiplication of CH virus in the presence of FUDR occurred in a few resistant cells in each culture and after successive cycles of infection the titers became as high as in virus controls.
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