Abstract
Summary
Some of the possible variables influencing the uptake of radioactive triiodo-thyronine from plasma by red blood cells (RBC) of dairy cattle were investigated. The in vitro uptake of T3-I131 by RBC was strikingly increased when incubation was carried out in tubes flushed with CO2. The antibiotic treatment and storage of blood at 54dGC prior to incubation delayed but did not prevent the decline in uptake of T3-I131 by RBC upon storage. No significant difference was found in the uptake of blood samples stored for zero and one day. Uniform uptake values were found by extending the storage time of T3-I131 up to 51/2 weeks prior to use on uptake of T3-I131 by RBC. The lot of T3-I131 stored for 71/2 weeks indicated a chemical breakdown of the triiodothyronine since a significant drop in its uptake was found. Within the levels studied, the changed concentration of exogenous tracer levels of T3 was found to have no significant effect upon uptake of T3-I131.
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