Abstract
Summary
Spleen cell nuclei, mitochondria, ribunucleoprotein and deoxyribonucleoprotein-rich fractions have been prepared from immunologically unresponsive NIH mice which had received a large inoculum of Shigella soluble antigen at birth. Presence of Shigella agglutinogens in these subcellular fractions has been inferred by use of an indirect method in which the fractions were incubated with fresh spleen cell suspensions, followed by transfer to new recipient mice. A rapid rise in specific anti-Shigella agglutinins in these recipients suggested the presence of Shigella agglutinogens in the original fractions. Such agglutinogenic activity was detected in most fractions for 2 to 4 weeks following neonatal injection, whereas ribonucleoprotein fractions exhibited detectable agglutinogenic activity for 6 to 8 weeks.
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