Use of an Antiserum Against Cerebrospinal Fluid in Demonstration of Trace Proteins in Biological Fluids. ‡

Abstract

Summary

Rabbit antisera to human cerebrospinal fluid, after “absorption” with human serum, have been shown to react with various human biological fluids. The reactions have been studied by immunoelectrophoresis and double diffusion in agar, and have revealed the presence of 2 trace proteins of β and γ electrophoretic mobility. Both proteins, “β_trace”- and “γ_trace”-, were found in CSF, urine and ascitic fluid, while the γ_trace-protein was also demonstrated in serum and pleural fluid. Exhaustive absorption of the antiserum to CSF with human serum removed the antibody to the γ_trace-protein.