Abstract
Summary
Concentration of ESF in the urine of Thalassemia patients increases with severity of anemia. Subjects with hemoglobin levels below 4 g % may reasonably be expected to provide urine with a high titre of human ESF. A biological assay depending upon the single unit obtained by the additive changes in hematocrit and reticulocyte values of rats has been developed which produces a cheaper and more reliable method of screening samples for ESF activity than the starved rat red cell Fe50 uptake procedure. The latter method, however, is more sensitive and there-fore more useful in following the purification of ESF preparations of established potency.
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