Abstract
Summary
A rapid chemical method for detection of insulin-binding antibodies and separation of free and antibody-bound insulin in human antiserums is described. With NaCl-saturated 76% ethanol solution in 0.01 M KOH at −12°C to −10°C, 87% − 100% of antibody-bound insulin was precipitated from 15 of 18 insulin antiserums whereas 85% − 94% of unbound insulin in control serums remained in solution.
The author is indebted to Drs. S. A. Berson and R. S. Yalow for aid and encouragement and for supplying insulin-I131 and serums. Thanks are also due Frieda Steiner for secretarial assistance.
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