Inosine Effects on Plasma Inorganic Phosphate Movements in Fresh and Gold-Stored Blood. ∗

Abstract

Summary

Movements of P³²O₄ in and out of fresh and cold-stored human erythrocytes were measured in presence and absence of inosine. I. Small amounts of hemolysis when inosine was present caused a decrease in plasma inorganic phosphate and incorporation of P³² into plasma organic phosphate. This formation of plasma organic phosphate decreased movement of P³² into the cell. II. When P³² was originally present in the red cell, inosine decreased the net movement from cells to plasma. We attribute this effect to utilization of inosine as substrate in the cell. III. Cells containing P³² during 6-day cold-storage lost 3 to 4 times as much P³² as cells having P³² for a short time. Movements from cells to plasma are described as the result of at least 2 phosphate compartments within the cell.