The control of the angle of incidence in attenuated total reflection (ATR) Fourier transform infrared (FT-IR) spectroscopy allows for the probing of the sample at different depths of penetration of the evanescent wave. This approach has been recently coupled with macro-imaging capability using a diamond ATR accessory. In this paper, the design of optical apertures for the micro-germanium (Ge) ATR objective is presented for an FT-IR spectroscopic imaging microscope, allowing measurements with different angles of incidence. This approach provides the possibility of three-dimensional (3D) profiling in micro-ATR FT-IR imaging mode. The proof of principle results for measurements of polymer laminate samples at different angles of incidence confirm that controlling the depth of penetration is possible using a Ge ATR objective with added apertures.
KazarianS.G.ChanK.L.A.. “Micro- and Macro-Attenuated Total Reflection Fourier Transform Infrared Spectroscopic Imaging”. Appl. Spectrosc.2010. 64(5): 135A–152A.
2.
GoormaghtighE.RaussensV.RuysschaertJ.. “Attenuated Total Reflection Infrared Spectroscopy of Proteins and Lipids in Biological Membranes”. Biochim. Biophys. Acta.1999. 1422(2): 105–185.
3.
OrsiniF.AmiD.VillaA.M.SalaG.BellottiM.G.DogliaS.M.“FT-IR Microspectroscopy for Microbiological Studies”. J. Microbiol. Methods.2000. 42(1): 17–27.
4.
PawlikowskiM.BenkoA.WróbelT.P.. “Degradation of Glycine and Alanine on Irradiated Quartz”. Orig. Life Evol. Biosph.2013. 43(2): 119–127. doi:10.1007/s11084-013-9328-3.
5.
KazarianS.G.ChanK.L.A.. “ATR-FTIR Spectroscopic Imaging: Recent Advances and Applications to Biological Systems”. Analyst. 2013. 138(7): 1940–1951. doi:10.1039/c3an36865c.
6.
KuimovaM.K.ChanK.L.A.KazarianS.G.. “Chemical Imaging of Live Cancer Cells in the Natural Aqueous Environment”. Appl. Spectrosc.2009. 63(2): 164–171. doi:10.1366/000370209787391969.
7.
WrobelT.P.MarzecK.M.MajznerK.KochanK.BartusM.ChlopickiS.BaranskaM.. “Attenuated Total Reflection Fourier Transform Infrared (ATR-FTIR) Spectroscopy of a Single Endothelial Cell”. Analyst. 2012. 137(18): 4135–4139. doi:10.1039/c2an35331h.
8.
MarzecK.M.WrobelT.P.RygulaA.MaslakE.JasztalA.FedorowiczA.ChlopickiS.BaranskaM.. “Visualization of the Biochemical Markers of Atherosclerotic Plaque with the Use of Raman, IR and AFM”. J. Biophotonics.2014. 7(9): 744–756. doi:10.1002/jbio.201400014.
9.
VoronkoY.ChernevB.S.EderG.C.. “Spectroscopic Investigations on Thin Adhesive Layers in Multi-Material Laminates”. Appl. Spectrosc.2014. 68(5): 584–592. doi:10.1366/13-07291.
10.
KaszowskaZ.MalekK.PańczykM.MikoajskaA.. “A Joint Application of ATR-FTIR and SEM Imaging with High Spatial Resolution: Identification and Distribution of Painting Materials and Their Degradation Products in Paint Cross Sections”. Vib. Spectrosc.2013. 65: 1–11. doi:10.1016/j.vibspec.2012.11.018.
SpringM.RicciC.PeggieD.KazarianS.G.. “ATR-FTIR Imaging for the Analysis of Organic Materials in Paint Cross Sections: Case Studies on Paint Samples from the National Gallery, London”. Anal. Bioanal. Chem.2008. 392(1–2): 37–45. doi:10.1007/s00216-008-2092-y.
13.
NagleD.J.GeorgeG.A.RintoulL.FredericksP.M.. “Use of Micro-ATR FT-IR Imaging to Study Heterogeneous Polymer Oxidation by Direct Solvent Casting onto the ATR IRE”. Vib. Spectrosc.2010. 53(1): 24–27. doi:10.1016/j.vibspec.2010.01.018.
14.
ChanK.L.A.KazarianS.G.. “Attenuated Total Reflection Fourier Transform Infrared Imaging with Variable Angles of Incidence: A Three-Dimensional Profiling of Heterogeneous Materials”. Appl. Spectrosc.2007. 61(1): 48–54. doi:10.1366/000370207779701415.
15.
ChanK.L.A.TayF.H.PoulterG.KazarianS.G.. “Chemical Imaging with Variable Angles of Incidence Using a Diamond Attenuated Total Reflection Accessory”. Appl. Spectrosc.2008. 62(10): 1102–1107. doi:10.1366/000370208786049222.
16.
FroschT.ChanK.L.A.WongH.C.CabralJ.T.KazarianS.G.. “Nondestructive Three-Dimensional Analysis of Layered Polymer Structures with Chemical Imaging”. Langmuir. 2010. 26(24): 19027–19032. doi:10.1021/la103683h.
17.
TayF.H.KazarianS.G.. “Study of Petroleum Heat-Exchanger Deposits with ATR FT-IR Spectroscopic Imaging”. Energy Fuels. 2009. 23(8): 4059–4067.
18.
DouganJ.A.KazarianS.G.. “Fourier Transform Infrared Spectroscopic Imaging of Live Cells”. Spectrosc. Eur.2013. 25(5): 6–12.
19.
WrobelT.P.KazarianS.G.BaranskaM.. “Apertura Optyczna do wyboru Kata Padania W Obiektywach Typu Cassegrain”/“Optical Apertures to Select the Angle of Incidence in Cassegrain Type Objectives”. Poland Patent 405822. Filed2014.
20.
HarrickN.J.Internal Reflection Spectroscopy. New York: Interscience Publishers, 1967. 3rd ed.
21.
NodaI.OzakiY.. Two-Dimensional Correlation Spectroscopy: Applications in Vibrational and Optical Spectroscopy. Chichester, West Sussex: John Wiley and Sons, 2005.
