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Abstract

Infrared (IR) spectroscopy has been successfully applied to study the permeation of substances through human skin in a high-throughput manner. The sample of skin was placed on the measuring surface of an attenuated total reflection (ATR) crystal and was divided into several areas. These areas were separated using a specially designed grid created on the surface of the skin and each area was subjected to a different combination of permeant and enhancer. ATR Fourier transform infrared (FT-IR) imaging was applied to measure the permeation of 12 liquid samples through a piece of skin smaller than 5 cm². This work demonstrated that, using the ATR-FT-IR imaging method, it is possible to measure and directly compare the transdermal processes of several permeants under identical conditions.

Keywords

High throughput Drugs Human skin Diffusion Stratum corneum Imaging Attenuated total reflection Fourier transform infrared spectroscopy ATR-FT-IR imaging

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High-Throughput Spectroscopic Imaging Applied to Permeation through the Skin

Abstract

Keywords

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