Developing a noninvasive method to assess the degraded state of historical parchments is essential to providing the best possible care for these documents. The conformational changes observed when collagen molecules, the primary constituent of parchment, unfold have been analyzed using attenuated total reflection–Fourier transform infrared (ATR-FT-IR) spectroscopy and the nanoscopic structural changes have been analyzed using X-ray diffraction (XRD). The relationship between the results obtained from these techniques was studied using principal component analysis, where correlation was found. The extent of gelatinization of historical parchments has been assessed using ATR-FT-IR and XRD and the frequency shifts observed as collagen degrades into gelatin have been reported. These results indicate that collagen degradation can be measured noninvasively in parchment and demonstrate the utility of ATR-FT-IR spectroscopy as a method to investigate historical documents.
LarsenR. Ed., Microanalysis of Parchment (Archetype Publications Ltd, London, 2002).
2.
KennedyC. J. and WessT. J, Restaurator–Int. J. Preserv. Library Archival Mater.24, 61 (2003).
3.
LaurentG. J., Am. J. Physiol. Cell Physiol.252, 1 (1987).
4.
DolginB.BulatovV. and SchechterI, Anal. Bioanal. Chem.388, 1885 (2007).
5.
BornsteinP. and SageH, Annu. Rev. Biochem.49, 957 (1980).
6.
FessasD.SignorelliM.SchiraldiA.KennedyC. J.WessT. J.HasselB. and NielsonK, Thermochim. Acta447, 30 (2006).
7.
LarsenR.PoulsenD. V.VestM. and JensenA. L, “Amino Acid Analysis of New and Historical Parchments”, in Microanalysis of Parchment, LarsenR. Ed. (Archetype Publications Ltd, London, 2002), Chap. 9, p. 93.
8.
JuchauldF. and ChahineC, “Determination of the Molecular Weight Distribution in Parchment Collagen by Steric Exclusion Chromatography”, in Microanalysis of Parchment, LarsenR. Ed. (Archetype Publications Ltd, London, 2002), Chap. 13, p. 123.
9.
PaceC. N.ShirleyB. A.McNuttM. and GajiwalaK, FASEB J.10, 75 (1996).
10.
MaxwellC. A.BellN.KennedyC. J. and WessT. J, The Paper Conservator29, 55 (2006).
11.
DerrickM., The Book and Paper Group, The American Institute for Conservation10 (1991).
12.
PaviaD. L.LampmanG. and KrizG. S, Spectroscopy (Saunders College Publishing, New York, 1996), 2nd ed.
13.
WessT. J.WessL. and HockingP. M, J. Comparative Pathol.116, 145 (1997).
14.
IconomidouV.GeorgakaM. E.ChryssikosG. D.GionisV.MegalofonouP. and HamodrakosS, Int. J. Biol. Macromol.41, 102 (2007).
15.
RabinI. and FranzkaS, “Microscopy and Parchment Degradation: A Comparative Study”, in Bio- and Material Cultures at Qumran, GunnewegJ.GreenblattC. and AdriaensA, Eds. (Jerusalem, Israel, 2005).
16.
JolliffeI. T., Principal Component Analysis (Springer, New York, 2002).
17.
GardinerW. P., Statistical Analysis Methods for Chemists: A Software Based Approach (The Royal Society of Chemistry, London, 1997).
18.
MantschH. H. and ChapmanD, Infrared Spectroscopy of Biomolecules (Wiley-Liss Inc, New York, 1996).
19.
YakimetsI.WellnerN.SmithA. C.WilsonR. H.FarhatI. and MitchellJ, Polymer46, 12577 (2005).
20.
Doyle-BrodskyB.BenditE. G. and BloutE. R, Biopolymers14, 937 (1975).
21.
LiH.ChenH. L.LuoR.LiuB. L. and GaoL. Z, Macromol. Biosci.3, 344 (2003).
22.
RenugopalakrishnanV.ChandrakasanG.MooreS.HutsonT.BerneyC. V. and BhatnagarR. S, Macromolecules22, 4121 (1989).
23.
GhioniC.HillerJ. C.KennedyC. J.AlievA. E.OdlyhaM.BoultonM. and WessT. J, J. Lipid Res.46, 2726 (2005).
24.
MantschH. H. and JacksonM, J. Mol. Struct.347, 187 (1995).
25.
TorikaiA. and ShibataH, J. Appl. Polym. Sci.73, 1259 (1998).
26.
LiuY.ChoR. K.SakuriK.MiuraT. and OzakiY, Appl. Spectrosc.48, 1249 (1994).
27.
RobinsonG. W. and ChoC. H, Biophys. J.77, 3311 (1999).
28.
DerkachS. R.DyakinaT. A. and LevachevS. M, Colloid J.67, 547 (2005).
29.
SurewiczW. K.MantschH. H. and ChapmanD, Biochemistry32, 389 (1993).
30.
HiramatsuH. and KitagawaT, Biochim. Biophys. Acta1753, 100 (2005).
31.
BigiA.CojazziG.RoveriN. and KochM. H. J, Int. J. Biol. Macromol.9, 363 (1987).
32.
BigiA.GandolfiM.KochM. H. J. and RoveriN, Biomaterials17, 1195 (1996).
33.
EikenberryE. F. and BrodskyB, J. Mol. Biol.144, 397 (1980).
34.
KennedyC. J.HillerJ. C.OdlyhaM.NielsonK.DrakopoulosM. and WessT. J, Papier Restaurierung3, 23 (2002).
35.
MeekK. M. and QuantockA. J, Prog. Retinal Eye Res.20, 95 (2001).
36.
PezronI.DjabourovM.BosioL. and LeblondJ, J. Polym. Sci. Part B: Polym. Phys.28, 1823 (1990).
37.
WessT. J. and OrgelJ. P, Thermochim. Acta365, 119 (2000).
38.
FraserR. D. B.MacRaeT. P. and SuzukiE, J. Mol. Biol.129, 463 (1979).
39.
BrodskyB.EikenberryE. F. and CassidyK, Biochim. Biophys. Acta621, 162 (1980).
40.
HulmesD. J. S. and MillerA, Nature (London)282, 878 (1979).
41.
MaxwellC. A.WessT. J. and KennedyC. J, Biomacromolecules7, 2321 (2006).
42.
DickY. P. and NordwigA, Arch. Biochem. Biophys.117, 466 (1966).
43.
BurjanadzeT. V. and KisiriyaE. L, Biopolymers21, 1695 (1982).
