Abstract
Fourier transform infrared (FT-IR) spectra have been measured for defatted bovine serum albumin (BSA) in D2O with a concentration of 2.0 wt % over a temperature range of 26–90 °C and the corresponding difference spectra have been calculated by subtracting the contribution of D2O at the same temperature. Evolving factor analysis (EFA) by selecting two factors and three factors has been employed to analyze the temperature-dependent difference IR spectra in the 1700–1600 cm−1 spectral region of the defatted BSA in D2O solution. Three-factor EFA has been employed to determine the distinction of the three protein species involved in the process of temperature elevation: native, transitional, and denatured protein. The temperature profiles obtained from three-factor EFA indicate that heat-induced conformational change in the secondary structures of defatted BSA in D2O undergoes two two-state transitions, a drastic transition and a slight transition, which occur in the temperature ranges of 68–82 °C and 56–76 °C, respectively.
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References
1.
Clark
R. J. H.
Hester
R. E.
, Eds., Biomolecular Spectroscopy (John Wiley and Sons , Chichester , 1993 ), Vol. 20 , part A, and vol. 21 , part B.
2.
Havel
H. A.
, Spectroscopic Methods for Determining Protein Structure in Solution (John Wiley and Sons , Chichester , 1995 ).
3.
Jackson
M.
Mantsch
H. H.
, Crit. Rev. Biochem. Mol. Biol. 20 , 95 (1995 ).
4.
Mantsch
H. H.
Chapman
D.
, Eds., Infrared Spectroscopy of Biomolecules (John Wiley and Sons , New York , 1996 ).
5.
Jackson
M.
Sowa
M. G.
Mantsch
H. H.
, Biophys. Chem. 68 (1–3 ), 109 (1997 ).
6.
Arrondo
J. L. R.
Goni
F. M.
, Prog. Biophys. Mol. Biol. 72 , 367 (1999 ).
7.
Byler
D. M.
Susi
H.
, Biopolymers 25 , 469 (1986 ).
8.
Dong
A.
Huang
P.
Caughey
W. S.
, Biochemistry 29 , 3303 (1990 ).
9.
Dousseau
F.
Pézolet
M.
, Biochemistry 29 , 8771 (1990 ).
10.
Fu
F. N.
DeOliveira
D. B.
Trumble
W. R.
Sarkar
H. K.
Singh
B. R.
, Appl. Spectrosc. 48 , 1432 (1994 ).
11.
Sungsool
W. I.
Petr
P.
Timothy
A. K.
, Biospectroscopy 4 , 93 (1998 ).
12.
Manas
E. S.
Getahun
Z.
Wright
W. W.
DeGrado
W. F.
Vanderkooi
J. M.
, J. Am. Chem. Soc. 122 , 9883 (2000 ).
13.
Weert
M.
Haris
P. I.
Hennink
W. E.
Crommelin
D. J. A.
, Anal. Biochem. 297 , 160 (2001 ).
14.
Oberg
K. A.
Ruysschaert
J. M.
Goormaghtigh
E.
, Eur. J. Biochem. 271 , 2937 (2004 ).
15.
Militello
V.
Casarino
C.
Emanuele
A.
Giostra
A.
Pullara
F.
Leone
M.
, Biophys. Chem. 107 , 175 (2004 ).
16.
Murayama
K.
Tomida
M.
, Biochemistry 43 , 11526 (2004 ).
17.
Noda
I.
Ozaki
Y.
, Appl. Spectrosc. 54 , 236A (2000 ).
18.
Jung
Y. M.
Czarnik-Matusewicz
B.
Ozaki
Y.
, J. Phys. Chem. B 104 , 7812 (2000 ).
19.
Schultz
C. P.
Bârzu
O.
Mantsch
H. H.
, Appl. Spectrosc. 54 , 931 (2000 ).
20.
Murayama
K.
Wu
Y.
Czarnik-Matusewicz
B.
Ozaki
Y.
, J. Phys. Chem. B 105 , 4763 (2001 ).
21.
Wu
Y. Q.
Ozaki
Y.
, J. Infrared Milim. Waves 22 , 161 (2003 ).
22.
Ozaki
Y.
Murayama
K.
Wu
Y.
Czarnik-Matusewicz
B.
, Spectroscopy 17 , 79 (2003 ).
23.
Yuan
B.
Zhao
H. Y.
Huang
M. Z.
Dou
X. M.
, J. Infrared Milim. Waves 23 , 213 (2004 ).
24.
Dou
X.
Yuan
B.
Zhao
H.
Yin
G.
Ozaki
Y.
, Sci. China, Ser. B 47 , 257 (2004 ).
25.
Zhang
J.
He
H. W.
Wang
Q.
Yan
Y. B.
, Protein Peptide Lett. 13 , 33 (2006 ).
26.
Kim
Y. J.
Yoon
G.
, Appl. Spectrosc. 56 , 625 (2002 ).
27.
De Juan
A.
Tauler
R.
, Anal. Chim. Acta 500 , 195 (2003 ).
28.
Ishiguro
T.
Ono
T.
Nakasato
K.
Tsukamoto
C.
Shimada
S.
, Biosci. Biotechnol. Biochem. 67 , 752 (2003 ).
29.
Etzion
Y.
Linker
R.
Cogan
U.
Shmulevich
I.
, J. Dairy Sci. 87 , 2779 (2004 ).
30.
Lam
H. S.
Proctor
A.
Nyalala
J.
Morris
M. D.
Smith
W. G.
, Lipids 39 , 687 (2004 ).
31.
Navea
S.
Tauler
R.
Goormaghtigh
E.
De Juan
A.
, Proteins 63 , 527 (2006 ).
32.
Moros
J.
Inon
F. A.
Khanmohammadi
M.
De Juan
A.
, Anal. Bioanal. Chem. 385 , 708 (2006 ).
33.
Gampp
H.
Maeder
M.
Meyer
C. J.
Zuberbuler
A. D.
, Talanta 32 , 1133 (1985 ).
34.
Maeder
M.
, Anal. Chem. 59 , 527 (1987 ).
35.
Tauler
R.
Casassas
E.
, J. Chemom. 3 , 151 (1988 ).
36.
Gemperline
P.
Hamilton
J. C.
, J. Chemom. 3 , 455 (1988 ).
37.
Keller
H. R.
Massart
D. L.
, Chemom. Intell. Lab. Syst. 12 , 209 (1992 ).
38.
Machado
A. A. S. C.
da Silva
J. C. G. E.
, Chemom. Intell. Lab. Syst. 17 , 249 (1992 ).
39.
Silva
C. S. P. C. O.
da Silva
J. C. G. E.
Machado
A. A. S. C.
, Appl. Spectrosc. 48 , 363 (1994 ).
40.
da Silva
J. C. G. E.
Machado
A. A. S. C.
, Chemom. Intell. Lab. Syst. 27 , 115 (1995 ).
41.
Bell
W. C.
Booksh
K. S.
Myrick
M. L.
, Anal. Chem. 70 , 332 (1998 ).
42.
Lizuka
K.
Aishima
T.
, J. Food Sci. 64 , 973 (1999 ).
43.
Nicholoson
M. A.
Aust
J. F.
Booksh
K. S.
Bell
W. C.
Myrick
M. L.
, Vib. Spectrosc. 24 , 157 (2000 ).
44.
Yuan
B.
Murayama
K.
Wu
Y. Q.
Tsenkova
R.
Dou
X. M.
Era
S.
Ozaki
Y.
, Appl. Spectrosc. 57 , 1223 (2003 ).
45.
Navea
S.
De Juan
A.
Tauler
R.
, Anal. Chem. 75 , 5592 (2003 ).
46.
Ruckebusch
C.
Duponchel
L.
Huvenne
J. P.
Saurina
J.
, Vib. Spectrosc. 35 , 21 (2004 ).
47.
Malinowaski
E. R.
Howary
D. G.
, Factor Analysis in Chemistry (Wiley-Intersicience , New York , 2002 ), 3rd ed.
48.
Jiang
J. H.
Liang
Y.
Ozaki
Y.
, Chemom. Intell. Lab. Syst. 71 , 1 (2004 ).
49.
Zhao
Z.
Malinowski
E. R.
, J. Chemom. 13 , 83 (1999 ).
50.
Peters
T.
Jr.
, All About Albumin (Academic Press , New York , 1994 ).
51.
Itoh
T.
Wada
Y.
Nakanishi
T.
, Agric. Biol. Chem. 40 , 1083 (1976 ).
52.
Ruegg
M.
Moor
U.
Blanc
B.
, J. Dairy Res. 44 , 509 (1977 ).
53.
Michnik
A.
, J. Therm. Anal. Calorim. 71 , 509 (2003 ).
54.
Harmsen
B. J. M.
Braam
W. J. M.
, Int. J. Protein Res. 1 , 255 (1969 ).
55.
Lin
V. J. C.
Koenig
J. L.
, Biopolymers 15 , 203 (1976 ).
56.
Clark
A. H.
Saunderson
D. H. P.
Suggett
A.
, Int. J. Peptide Protein Res. 17 , 353 (1981 ).
57.
Sogami
M.
Foster
J. F.
, Biochemistry 7 , 2172 (1968 ).
58.
Fabian
H.
Schultz
C.
Naumann
D.
Landt
O.
Hahn
U.
Saenger
W.
, J. Mol. Biol. 232 , 967 (1993 ).
59.
Fabian
H.
Shultz
C.
Backmann
J.
Hahn
U.
Saenger
W.
Mantsch
H. H.
Naumann
D.
, Biochemistry 33 , 10725 (1994 ).
60.
Kauppinen
J. K.
Mofatt
D. J.
Mantsch
H. H.
Cameron
D. G.
, Appl. Spectrosc. 35 , 271 (1981 ).