Application of mid-infrared spectroscopy for the determination of urea in blood plasma dialysates of microliter sample volumes using a transmission microcell was investigated. Infrared spectra of the dialysates of plasma samples collected from 75 different patients using CMA 60 microdialysis catheters were evaluated with multivariate partial least squares regression. Using the absorbance spectral data from 1520–1420 cm−1 and 1220–1120 cm−1, a minimum standard error of prediction (SEP) of 0.88 mg/dL (0.14 mM) was achieved with spectral variable selection. Our findings suggest the feasibility of developing a mid-infrared sensor in combination with micro-fluidics for on-line monitoring of urea in patients undergoing dialysis treatment.
HeiseH. M., “Clinical Applications of Near and Mid-Infrared Spectroscopy”, in Infrared and Raman Spectroscopy of Biological Materials, GremlichH. U. and YanB, Eds. (Marcel Dekker, Inc., New York, 2001), Chap. 8, p. 259.
2.
LowrieE. G.LairdN. M.ParkerT. F. and SargentJ. A, N. Engl. J. Med.305, 1176 (1981).
FernandezJ. M.CarbonellM. E.MazzuchiN. and PetruccelliD, Kidney Int.41, 1029 (1992).
6.
HakimR. M.BreyerJ.IsmailN. and SchulmanG, Am. J. Kidney Dis.23, 661 (1994).
7.
HeldP. J.PortF. K.WolfeR. A.StannardD. C.CarrollC. E.DaugirdasJ. T.GreerJ. W. and HakimR. M, Kidney Int.50, 550 (1996).
8.
NolphK. D.TwardowskiZ. J. and KeshaviahP. R, Perit. Dial. Int.12, 298 (1992).
9.
JindalK. K.ManuelA. and GoldsteinM. B, ASAIO Trans.33, 286 (1987).
10.
BasileC.CasinoF. and LopezT, Am. J. Kidney Dis.15, 40 (1990).
11.
FlaniganM. J.FangmanJ. and LimV. S, Am. J. Kidney Dis.17, 295 (1991).
12.
BankheadM. M.TotoR. D. and StarR. A, Kidney Int.48, 785 (1995).
13.
CharytanC.GuptaB.MeindelN. and SpinowitzB, Kidney Int.50, 1845 (1996).
14.
RajD. S.TobeS.SaiphooC. and ManuelM. A, Int. J. Artif. Organs20, 422 (1997).
15.
EisenbergE. J. and EickhoffW. M, J. Pharmacol. Toxicol. Methods30, 27 (1993).
16.
ChenJ.ArnoldM. A. and SmallG. W, Anal. Chem.76, 5405 (2004).
17.
ShawR. A.KotowichS.MantschH. H. and LerouxM, Clin. Biochem.29, 11 (1996).
18.
PezzanitiJ. L.JengT. W.McDowellL. and OostaG. M, Clin. Biochem.34, 239 (2001).
19.
EddyC. V. and ArnoldM. A, Clin. Chem.47, 1279 (2001).
20.
EddyC. V.FlaniganM. and ArnoldM. A, Appl. Spectrosc.57, 1230 (2003).
21.
OlesbergJ. T.ArnoldM. A. and FlaniganM. J, Clin. Chem.50, 175 (2004).
22.
JensenP. S.BakJ.LadefogedS.Andersson-EngelsS. and Friis-HansenL, J. Biomed. Opt.9, 553 (2004).
23.
HeiseH. M.VoigtG.LampenP.KüpperL.RudloffS. and WernerG, Appl. Spectrosc.55, 434 (2001).
24.
HeiseH. M.MarbachR.KoschinskyT. H. and GriesF. A, Appl. Spectrosc.48, 85 (1994).
25.
RohlederD.KocherscheidtG.GerberK.KieferW.KöhlerW.MöcksJ. and PetrichW, J. Biomed. Opt.10, 031108 (2005).
26.
JensenP. S.BakJ.LadefogedS. and Andersson-EngelsS, Spectrochim. Acta, Part A60, 899 (2004).
27.
HeiseH. M. and BittnerA, Fresenius' J. Anal. Chem.359, 93 (1997).
28.
HeiseH. M.KüpperL. and ButvinaL. N, Spectrochim. Acta, Part B57, 1649 (2002).
29.
MartinW. B.MirowS. and VenugopalanR, Appl. Spectrosc.59, 881 (2005).
30.
HeiseH. M.DammU.VogtO. and KondepatiV. R, “Towards Reagent-Free Blood Glucose Monitoring Using Micro-Dialysis and Infrared Transmission Spectrometry”, Vib. Spectrosc., paper in press (2006).
31.
HeiseH. M.DammU. and KondepatiV. R, Proc. SPIE-Int. Soc. Opt. Eng.6093, 21 (2006).
32.
DammU.KondepatiV. R. and HeiseH. M, “Continuous Reagent-Free Bed-Side Monitoring of Glucose in Biofluids Using Infrared Spectrometry and Micro-Dialysis”, Vib. Spectrosc., paper submitted (2006).
