We have measured jugular venous oxygen saturation (sjvO2) and lactate, arterial and jugular venous blood levels of lipid peroxidation products - malondialdehyde (MDA) and diene conjugates as an index of free radical activity in eight adults undergoing coronary artery bypass surgery. Measurements were carried out at six specific times: T1 - bypass (CPB), T2 - within 5 min before cardiopulmonary within the first minute after the commencement of CPB, T3 - during stable temperature (28°C) on CPB, T4 - during rewarming at 34°C, T5 min after CPB and T6 - at skin closure. There were no significant changes in arterial, jugular venous and arterio-jugular venous (a-jv) differences in diene conjugates and MDA. There was no correlation between sjvO2, lactate and a-jv differences in MDA and diene conjugates. These results are not indicative of ischaemia-reperfusion injury across the cerebral circulation during hypothermic CPB.
Get full access to this article
View all access options for this article.
References
1.
Shaw PJ , Bates D , Cartlidge NEFet al.Neurologic and neuropsychological morbidity following major surgery: comparison of coronary artery bypass and peripheral vascular surgery . Stroke1987; 17: 700-707 .
2.
Ip-Yam PC , Thomas SD , Jackson M , Rashid A , Behl S.Effects of temperature strategy during cardiopulmonary bypass on cerebral oxygen balance . J Cardiovasc Surg2000; 41: 1-6 .
3.
Iversen SA , Cawood P , Dormandy TL.A method for the measurement of a diene-conjugated derivative of linoleic acid, 18:2(9,11), in serum phospholipids, and possible origins . Ann Clin Biochem1985; 22: 137-140 .
4.
Yagi K.A simple fluorometric assay for lipid peroxidation in blood serum or plasma. In CRC handbook of free radical and antioxidants in biomedicine, Volume III. Ohio: The Chemical Rubber Co , 1989: 215-217.
5.
Grech ED , Bellamy CM , Jackson MJ , Muirhead RA , Faragher EB , Ramsdale DR.Free-radical activity after primary coronary angioplasty in acute myocardial infarction . Am Heart J1994; 127: 1443-1449 .
6.
Croughwell ND , Frasco P , Blumenthal JA , Leone BJ , White WD , Reves JG.Warming during cardiopulmonary bypass is associated with jugular bulb desaturation . Ann Thorac Surg1992; 53: 827-832 .
7.
Valen G , Khoschorur GA , Almdahl SM , Esterbauer H , Vaage J.Lipid peroxidation in open-heart surgery . Perfusion1994; 9: 277-283 .
8.
Tortolani AJ , Powell SR , Misik V , Weglicki WB , Pogo CJ , Kramer JH.Detection of aloxyl and carbon-centered free radicals in coronary sinus blood from patients undergoing elective cardioplegia . Free Radic Biol Med1993; 14: 421-426 .
9.
Zini I , Tomasi A , Grimaldi R , Vannini V , Agnati LF.Detection of free radicals during brain ischemia and reperfusion by spin trapping and microdialysis . Neurosci Lett1992; 138: 279-282 .
