Novel calcium hydroxide/nanohydroxyapatite composites for dental applications: in vitro study

Abstract

The physical, physicochemical and antimicrobial properties of novel cement type calcium hydroxide/nanohydroxyapatite composites were evaluated by measuring setting time, compressive strength, phase analysis, pH, water solubility and bacterial inhibition zone. Different cement pastes were obtained by mixing biphasic powders (comprising mixtures of calcium hydroxide and nanohydroxyapatite in various weight ratios) and ester of salicylic acid as liquid. Cements with different amounts of nanohydroxyapatite set at approximately 4-14 min and had a compressive strength of 8-16 MPa. The crystalline phases of the set cements were hydroxyapatite and calcium hydroxide. Finally, all composite samples showed antibacterial activity, and the larger zone of bacterial inhibition was observed in composites with higher amount of Ca(OH)₂.

Keywords

Hydroxyapatite Composite materials Antimicrobial Calcium hydroxide

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References

Ducheyne

and de Groot

: J. Biomed. Mater. Res., 1981, 15, 441–445.

Jarcho

: Chit Orthop. Relat. Res., 1981, 157, 259–278.

Hench

L. L.

: J. Am. Ceram. Soc., 1991, 74, 1487–1510.

Saboori

, Sheikhi

, Moztarzadeh

, Rabiee

, Hesaraki

, Tahriri

and Nezafati

: Adv. Appl. Ceram., 2009, 108, 155–161.

Bloebaum

R. D.

, Beeks

, Dorr

L. D.

, Savory

C. G.

, DuPont

J. A.

and Hofmann

A. A.

: Clin. Orthop., 1994, 298, 19–26.

Bloebaum

R. D.

, Zou

, Bachus

K. N.

, Shea

K. G.

, Hofmann

A. A.

and Dunn

H. K.

: Clin. Orthop., 1997, 338, 109–118.

Patrice

, Alexia

G. L.

and Salima

A. R.

: Biomaterials, 2004, 25, 2515–2524.

Alexia

G. L.

, Patrice

and Dominique

L. M.

: Biomaterials, 2004, 25, 5921–5927.

, Thou

, Huang

W. H.

, Wang

D. P.

, Zhang

L. Y.

and Li

H. F.

: J. Biomed Mater. Res. A, 2005, 74A, 156–163.

10.

Yin

M. Z.

, Han

Y. C.

, Dai

H. L.

and Li

S. P.

: J. Wuhan Univ. TechnoL, 2006, 21, 102–104.

11.

Beyer-Olsen

E. M.

and Orstavik

: Oral Surg. Oral Med. Oral PathoL, 1981, 51, 320–328.

12.

Rashid

, Shiba

, Mizuno

, Mouri

, Fujita

and Shinohara

: J. Endod., 2003, 29, 104–107.

13.

Estrela

and Holland

: J. AppL Oral Sci., 2003, 11, 269–282.

14.

Holland

, Pinheiro

C. E.

, Mello

, Nery

M. J.

and Souza

: J. Endodont., 1982, 8, 444 447.

15.

Greish

Y. E.

and Brown

P. W.

: J. Biomed Mater. Res. B, 2003, 67B, 632–637.

16.

Azaroff

L. A.

: ‘Elements of X-ray crystallography‘, 1968, New York, McGraw-Hill.

17.

Khan

A. S.

, Ahmed

, Edirisinghe

M. J.

, Wong

F. S. L.

and Rehman

I. U.

: Acta Biomater., 2008, 4, 1275–1287.

18.

Kalita

S. J.

and Verma

: Mater. Set Eng. C, 2010, C30, 295–303.

19.

Sato

, Sambito

M. A.

, Aslani

, Kalkhoran

N. M.

, Slamovich

E. B.

and Webster

Th. J.

: Biomaterials, 2006, 27, 2358–2369.

20.

, Huang

and Edirisinghe

: J. Mater. ScL Med, 2008, 19, 1545–1551.

21.

, Huang

, Ahmad

and Edirisinghe

: Biomed Mater. Eng., 2007, 17, 335–346.

22.

, Huang

and Edirisinghe

M. J.

: J. R Soc. Interface, 2008,5, 253–257.

23.

, Koller

, Huang

, Silvio

L. D.

, Renton

, Esat

, Bonfield

and Edirisinghe

: J. R Soc. Interface, 2010, 7, 189–197.

24.

Ahmad

, Rasekh

and Edirisinghe

: MacromoL Mater. Eng., 2010, 295, 315–319.

25.

Stanley

H. R.

and Lundy

: Oral Surg. Oral Med Oral Pathol, 1972, 34, 818–827.

26.

Tam

L. E.

, Pulver

, McComb

and Smith

D. C.

: Dent. Mater., 1989, 5, 145–149.

27.

Hesaraki

, Moztarzadeh

and Sharifi

: J. Biomed. Mater. Res. A, 2007, 83A, 80–87.

28.

Estrela

and Pesce

H. F.

: Braz. Dent. J., 1996, 7, 41–6.

29.

Siqueira

J. F.

, Lopes

H. P.

: Int. Endod. J., 1999, 32, 361–369.