Abstract
Background
The pathogenesis of psoriasis vulgaris is attributed to an immune dysregulation of T lymphocytes, and psoriatic plaques result from interactions between keratinocytes and T lymphocytes.
Objective
To decipher the impact of keratinocyte-T lymphocyte interactions on cell survival during active cutaneous psoriasis by using a new in vitro model of psoriasis.
Methods
Cocultures of psoriatic or healthy keratinocytes were performed with normal human blood T lymphocytes. Apoptosis was detected by flow cytometry after labeling cells with annexin V and propidium iodide.
Results
Psoriatic keratinocytes were significantly more efficient than healthy keratinocytes in enhancing T lymphocyte survival.
Conclusion
Our in vitro model is pertinent to understanding the immunopathogenesis of psoriasis and to discovering new pharmacological targets.
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