Osteomyelitis associated with Bartonella henselae infection in a young cat

Abstract

Case summary

A 1-year-old male intact domestic shorthair cat was evaluated for acute onset non-weightbearing left forelimb lameness and generalized peripheral lymphadenopathy. CT identified a monostotic aggressive bone lesion with an incomplete fracture of the left radial metaphysis. Bone aspirates yielded osteoblasts with minimal nuclear atypia. Abdominal ultrasound revealed a nodular spleen and lymphadenopathy; cytologically, both contained lymphoid hyperplasia. A urine histoplasma antigen test was negative. Bartonella henselae and Mycoplasma haemominutum DNA was amplified by PCR from peripheral blood. Indirect immunofluorescence documented strong B henselae immunoreactivity, with lower Bartonella vinsonii subspecies berkhoffii and Bartonella koehlerae antibody titers. After the administration of doxycycline and pradofloxacin for suspected Bartonella-induced osteomyelitis, lameness resolved rapidly. Six-week post-treatment radiographs identified healing of the affected bone, and Bartonella species enrichment blood culture was negative. B henselae antibody titers decreased four-fold over a year, supporting seroreversion.

Relevance and novel information

B henselae is a flea-transmitted, host-adapted species, not previously implicated as a cause of osteomyelitis in cats. B henselae subclinical bacteremia is highly prevalent among cats; however, bacteremia has been associated with lymphadenopathy and febrile illness in cats. This report describes a unique clinical presentation in association with B henselae infection in a cat.

Introduction

Bartonella species are Gram-negative, fastidious, facultative intracellular bacilli that have coevolved with various mammalian hosts, including cats, dogs and humans. ¹ Cats are the principal reservoir host for Bartonella henselae (Bh), Bartonella koehlerae (Bk) and Bartonella clarridgeiae (Bc).^1,2 B henselae has a global bacteremic prevalence as high as 50–76%.^1,3 Ctenocephalides felis is the principal arthropod vector for multiple Bartonella species (including Bh, Bk and Bc).^1,4,5 Disease expression following Bh infection is largely dependent on host immunocompetence, the presence of comorbidities or coinfections, and strain virulence. After infection with a host-adapted Bartonella species, healthy cats commonly experience subclinical bacteremia that persists for months to years.^1–3 Alternatively, mild or transient fever and lymphadenopathy are sometimes observed.^3,6 Serious disorders such as endocarditis, myocarditis, uveitis, encephalitis and musculoskeletal abnormalities are uncommonly reported.^1,3,7,8

Recognition of osteomyelitis as a manifestation of feline bartonellosis is hitherto limited to a case report detailing a domestic cat infected with non-host-adapted Bartonella vinsonii subspecies berkhoffii (Bvb). ⁹ Documentation of seroreversion (quantitative decrease in antibody titers) and its clinical significance during and after antimicrobial treatment for feline bartonellosis is also sparse. ⁶ We report the clinicopathologic findings in a cat with Bh bacteremia, osteomyelitis and serologic trends over a 1-year follow-up period.

Case description

A 1-year-old male intact domestic shorthair cat weighing 3 kg was referred for evaluation of an aggressive bone lesion of the left radius following a 4-week history of progressive left forelimb lameness. Left forelimb radiographs performed by the primary veterinarian revealed a small lucency in the left proximal radial metaphysis. There was minimal response to cage confinement and meloxicam (Metacam; Boehringer Ingelheim). The cat was infested with fleas. It had been introduced from a feral population into a multi-cat household 3 months before presentation.

Physical examination identified a non-weightbearing left forelimb lameness, a painful left elbow swelling and generalized peripheral lymphadenomegaly. Complete blood count demonstrated a mature neutrophilia (14,100/µl; reference interval [RI] 2500–12,500). Serum alkaline phosphatase was mildly elevated (54 U/l; RI 0–45). There was mild proteinuria (30 mg/dl) and poorly concentrated urine (cystocentesis performed after intravenous fluid therapy). Repeat radiographs confirmed the progression of an ill-defined lucency in the left proximal radial metaphysis, with thinning and discontinuity of the cranial aspect of the cortex with a short zone of transition (Figure 1). Circumferential irregular-to-smooth periosteal bone reaction and soft tissue swelling were evident. Abdominal ultrasound identified multifocal lymphadenopathy (up to 9.3 mm wide) and hypoechoic splenic nodules.

Figure 1

Orthogonal radiographs. (a) Mediolateral view and (b) craniocaudal view of the left elbow taken at the initial referral. Note the ill-defined, geographic lysis in the proximal metaphysis of the radius with focal cortical destruction and thinning (white arrows). These findings are consistent with an aggressive bone lesion from either osteomyelitis or neoplasia

Fine-needle aspiration of the affected bone yielded rare reactive osteoblasts. Cytologically, abdominal and peripheral lymph node aspirates contained reactive lymphoid hyperplasia. CT confirmed cortical lysis of the medial aspect of the left proximal radial metaphysis with irregular thinning of the cortex caudally and adjacent to the lytic region (Figure 2a), accompanied by a moderate amount of circumferential, smooth-to-brush border periosteal reaction (Figure 2b).

Figure 2

CT images (window length 550, window width 2700 in a bone algorithm with a 0.625 mm slice thickness) of the left proximal radius and ulna taken at the same time as the radiographs in Figure 1. (a) Transverse image of the left radius at the level of the proximal metaphyseal lytic area with cortical defect medially (white arrow). (b) Transverse image of the left radius distal to the lytic area seen in (a) showing the circumferential brush border new bone formation (white arrows)

Neoplasia was considered an unlikely differential diagnosis given the cat’s young age; therefore, additional infectious disease testing was pursued. A needle core bone biopsy was not attempted due to cortical lysis, small patient size and increased risk of an iatrogenic fracture. The owner declined surgical bone biopsy. Doxycycline (5 mg/kg PO q12h [Doxycycline monohyclate oral suspension USP; Cipla]) and clindamycin (16 mg/kg PO q12h [ClinDrops; Henry Shein Animal Health]) were initiated. Buprenorphine (0.02 mg/kg PO q8h [Buprenorphine hydrochloride; Par Sterile Products]) was weaned over 2 weeks.

Feline leukemia virus and feline immunodeficiency virus testing (SNAP FIV/FELV Combo; IDEXX Laboratories) was negative. Urine culture was negative for aerobic bacterial growth and urine was negative for histoplasma antigen (MiraVista Veterinary Diagnostics). A feline vector-borne infectious disease serology and PCR panel was submitted to the Vector Borne Diseases Diagnostic Laboratory, North Carolina State University. The Bh immunofluorescence assay (IFA) antibody titer was markedly elevated (1:4096); Bvb and Bk titers were 1:256 and 1:64, respectively. B henselae DNA was amplified from the cat’s blood (VB19-08571) specimen, using previously described Bartonella genus-specific ssrA real-time PCR (qPCR), 16S–23S intergenic transcribed spacer (ITS) region qPCR and 16S–23S ITS conventional PCR assays.^10,11 Blast analysis of conventional ITS sequences (National Center for Biotechnology Information Basic Local Alignment Search Tool) were 100% identical to Bh isolate MT095053.1 (569/569 base pairs) and 99.82% similar to Bh CAL-1 (GenBank accession No AF369527.1). B henselae CAL-1 has one extra nucleotide, an ‘A’, that was missing from the amplicon sequences obtained from this cat’s blood using both forward and reverse ITS primers. Mycoplasma haemominutum DNA was also amplified from the cat’s blood.

Based upon clinicopathological findings and infectious disease test results, osteomyelitis secondary to bartonellosis was suspected. Pradofloxacin oral suspension (7.5 mg/kg PO q24h [Veraflox; Bayer]) was initiated and doxycycline was continued. Clindamycin was discontinued. Two weeks later, the owner reported rapid improvement in weightbearing of the affected limb.

At re-evaluation 6 weeks later, the left forelimb lameness and peripheral lymphadenopathy had resolved. An echocardiogram, performed as a screening for Bartonella-induced endocarditis, was normal. There was resolution of the left elbow periosteal reaction, and decreased lysis of the proximal radial metaphysis, with smooth cortical bone remodeling (Figure 3). The radiographic findings, clinical response to antibiotics and previously documented microbiological results supported resolving osteomyelitis, presumptively secondary to bartonellosis. Bhenselae ssrA PCR was negative, indicating that bacteremia had resolved or was below the level of PCR detection. Indirect fluorescence antibody titers remained similarly elevated (Bh 1:2048; Bvb 1:256; Bk 1:256). Although the Bh titer decreased from 1:4096 to 1:2048, an increase or decrease in IFA titer by one dilution is generally interpreted as clinically and microbiologically unchanged. Although coinfection with more than one Bartonella species was possible, the serology and PCR results supported Bh bacteremia with potential Bvb and Bk cross-reactivity. M haemominutum DNA was again PCR amplified from the cat’s blood. Given the possibility of ongoing infection, particularly involving bone, antibiotic therapy with doxycycline and pradofloxacin was continued at the same dosages for another 6 weeks with the goals of complete elimination of Bartonella species bacteremia and documentation of seroreversion.

Figure 3

Orthogonal radiographs: (a) mediolateral view and (b) craniocaudal view taken 6 weeks after doxycycline and pradofloxacin administration. There was significant healing of the osteomyelitic lesion of the left radius. The lytic area in the proximal radial metaphysis and the cortical destruction were no longer noted with bone remodeling and resolution of the previously noted smooth-to-brush border new bone formation

During the subsequent 6 weeks, the cat remained clinically normal. Three months post-antibiotic therapy, radiographs illustrated complete resolution of the osteomyelitic lesion. Antibody titers (Bh 1:4096; Bvb 1:256; Bk 1:128) remained essentially unchanged. Persistent seroreactivity, in conjunction with negative Bartonella genus-specific ssrA qPCR, 16S–23S ITS region qPCR, PCR assays and resolution of clinical disease, was interpreted as a durable immune system memory response. Antibiotic therapy was discontinued. Ten months after diagnosis, the Bh IFA antibody titer was 1:1024 (four-fold decrease), Bvb was 1:16 and Bk was <1:16. Bartonella genus-specific ssrA qPCR, 16S–23S ITS region qPCR and PCR assays were again negative. Bartonella alpha proteobacteria growth medium (BAPGM) with qPCR testing ¹² at 7, 14 and 21 days was also negative. Twelve months after the onset of illness, the cat was outwardly healthy with no residual lameness.

Discussion

Osteomyelitis secondary to systemic bartonellosis is a rare manifestation of a common infection in cats. To our knowledge, Bh bacteremia has not previously been associated with feline osteomyelitis. Primary differential diagnoses for a monostotic osteolytic and osteoproliferative lesion involving the metaphysis include infectious osteomyelitis and neoplasia. Based on PCR positivity and high IFA seroreactivity, Bh was presumably the pathogen that caused osteomyelitis in this young cat. Although contributing roles from Bvb and Bk were possible, the late, negative convalescent serology results for these species supported Bh as the primary cause of osteomyelitis. While the cat remained bacteremic with M haemominutum despite antimicrobial treatment, this hemotropic Mycoplasma species is of relatively low pathogenicity and has not been implicated as a cause of osteomyelitis in cats. ⁴

Owing to the frequency of subclinical bartonellosis, proof of disease causation in bacteremic cats remains challenging. Diagnosis can be supported by positive blood or tissue culture, seroconversion or seroreversion in association with compatible clinical findings and visualization of the organism in association with a pathological lesion. ¹ While not widely documented in the literature, seroreversion in treated cats is noted in isolated reports, especially by use of Western blotting. ⁶ In this case, the combination of positive PCR, high Bh seroreactivity and rapid clinical improvement after the initiation of appropriate antibiotics and seroreversion provided very strong, albeit indirect, evidence for diagnosis of bartonellosis in this cat. Very elevated Bh titers often correlate with positive PCR or blood culture results in sick cats. ¹ In contrast, serology alone in chronically infected cats can be of low diagnostic yield as relapsing bacteremia has been documented in conjunction with low or non-detectable antibody titers, as also reported for dogs and humans.^1,2 Seroconversion in acute bartonellosis in combination with seroreversion with directed antibiotic therapy provides microbiological support for the diagnosis, as documented by sero-reversion in this cat. Following an acute illness, Bh and Bk seroconversion over a 2-month period was documented in a dog, followed by seroreversion with combination antimicrobial therapy. ¹³ In future feline cases, where osteomyelitis is suspected, obtaining aerobic, anaerobic and optimized Bartonella species enrichment cultures by aspiration or biopsy directly from the lesion would be recommended. Additionally, if biopsied, research laboratories can now visualize Bartonella species in diseased tissues using immunohistochemistry or in situ hybridization, which would provide more direct evidence of Bartonella species as a cause of osteomyelitis in cats.

Combination therapy with doxycycline and pradofloxacin should be considered in sick cats with systemic bartonellosis. ¹ This approach achieves high intracellular and plasma concentrations, increasing the chance of suppressing active bacteremia and/or attaining therapeutic elimination of intracellular bacteria. ¹ However, neither drug nor the combination of them guarantees infection cure.^1,3 Prolonged therapy with a minimum duration of 6 weeks has been recommended for clinical remission, if not bacterial eradication. Given the broad antimicrobial spectrum of doxycycline and pradofloxacin, a definitive diagnosis or a very high index of suspicion for bartonellosis is optimal before initiating treatment to avoid inducing antibiotic resistance. Treatment duration for bacterial osteomyelitis in dogs and cats is generally up to 8 weeks for oral antibiotic administration. ¹⁴ In this cat, there was incomplete radiographic resolution of the osteomyelitic lesion 6 weeks post-antibiotic administration, so a further 6 weeks of treatment was recommended. Three months post-initiation of antibiotic therapy, despite persistently elevated antibody titers, treatment was discontinued due to clinical and radiographic resolution. While dogs can exhibit a significant decrease in antibody titers 3–6 months after successful elimination of the Bh and Bvb, ⁵ the extent to which this holds true for cats is unclear. Therefore, sequential serology and BAPGM enrichment blood culture post-treatment were performed to assess completeness of pathogen elimination. Clinical remission and seroreversion, in conjunction with negative BAPGM enrichment blood culture results, provided support for therapeutic elimination of Bh in this cat.

Conclusions

Bartonellosis should be considered as a differential diagnosis in cats presenting with aggressive bone lesions and generalized lymphadenopathy.