Since the study of yeast RAS and adenylate cyclase in the early 1980s, yeasts including budding and fission yeasts contributed significantly to the study of Ras signaling. First, yeast studies provided insights into how Ras activates downstream signaling pathways. Second, yeast studies contributed to the identification and characterization of GAP and GEF proteins, key regulators of Ras. Finally, the study of yeast provided many important insights into the understanding of C-terminal processing and membrane association of Ras proteins.
Powers S,Kataoka T,Fasano O, et al.Genes in S. cerevisiae encoding proteins with domains homologous to the mammalian ras proteins.Cell. 1984;36:607-12
2.
DeFeo-Jones D,Scolnick EM,Koller R,Dhar R.ras-Related gene sequences identified and isolated from Saccharomyces cerevisiae.Nature. 1983;306:707-9
3.
Tamanoi F,Walsh M,Kataoka T,Wigler M.A product of yeast RAS2 is a guanine nucleotide binding protein.Proc Natl Acad Sci U S A. 1984;81:6924-8
4.
Breviario D,Hinnebusch A,Cannon J,Tatchell K,Dhar R.Carbon source regulation of RAS1 expression in Saccharomyces cerevisiae and the phenotypes of ras2- cells.Proc Natl Acad Sci U S A. 1986;83:4152-6
5.
DeFeo-Jones D,Tatchell K,Robinson LC, et al.Mammalian and yeast ras gene products: biological function in their heterologous systems.Science. 1985;228:179-84
6.
Kataoka T,Powers S,Cameron S, et al.Functional homology of mammalian and yeast RAS genes.Cell. 1985;40:19-26
7.
Fukui Y,Kaziro Y.Molecular cloning and sequence analysis of a ras gene from Schizosaccharomyces pombe.EMBO J. 1985;4:687-91
8.
Nadin-Davis SA,Yang RC,Narang SA,Nasim A.The cloning and characterization of a RAS gene from Schizosaccharomyces pombe.J Mol Evol. 1986;23:41-51
9.
Kataoka T,Powers S,McGill C, et al.Genetic analysis of yeast RAS1 and RAS2 genes.Cell. 1984;37:437-45
10.
Toda T,Uno I,Ishikawa T, et al.In yeast, RAS proteins are controlling elements of adenylate cyclase.Cell. 1985;40:27-36
11.
Field J,Vojtek A,Ballester R, et al.Cloning and characterization of CAP, the S. cerevisiae gene encoding the 70 kd adenylyl cyclase-associated protein.Cell. 1990;61:319-27
12.
Nikawa J,Sass P,Wigler M.Cloning and characterization of the low-affinity cyclic AMP phosphodiesterase gene of Saccharomyces cerevisiae.Mol Cell Biol. 1987;7:3629-36
13.
Sass P,Field J,Nikawa J,Toda T,Wigler M.Cloning and characterization of the high-affinity cAMP phosphodiesterase of Saccharomyces cerevisiae.Proc Natl Acad Sci U S A. 1986;83:9303-7
14.
Colombo S,Ma P,Cauwenberg L, et al.Involvement of distinct G-proteins, Gpa2 and Ras, in glucose- and intracellular acidification-induced cAMP signaling in the yeast Saccharomyces cerevisiae.EMBO J. 1998;17:3326-41
15.
Kido M,Shima F,Satoh T,Asato T,Kariya K,Kataoka T.Critical function of the Ras-associating domain as a primary Ras-binding site for regulation of Saccharomyces cerevisiae adenylyl cyclase.J Biol Chem. 2002;277:3117-23
16.
Sun J,Kale SP,Childress AM,Pinswasdi C,Jazwinski M.Divergent roles of RAS1 and RAS2 in yeast longevity.J Biol Chem. 1994;269:18638-45
17.
Ho J,Bretscher A.Ras regulates the polarity of the yeast actin cytoskeleton through the stress response pathway.Mol Biol Cell. 2001;12:1541
18.
McDonald CM,Wagner M,Dunham MJ,Shin ME,Ahmed NT,Winter E.The Ras/cAMP pathway and the CDK-like kinase Ime2 regulate the MAPK Smk1 and spore morphogenesis in Saccharomyces cerevisiae.Genetics. 2009;181:511-23
19.
Garrett JM.Amino acid transport through the Saccharomyces cerevisiae Gap1 permease is controlled by the Ras/cAMP pathway.Int J Biochem Cell Biol. 2008;40:496-502
20.
Wood MD,Sanchez Y.Deregulated Ras signaling compromises DNA damage checkpoint recovery in S. cerevisiae.Cell Cycle. 2010;9:3353-63
21.
Cobitz AR,Yim EH,Brown WR,Perou CM,Tamanoi F.Phosphorylation of RAS1 and RAS2 proteins in Saccharomyces cerevisiae.Proc Natl Acad Sci U S A. 1989;86:858-62
22.
Xiaojia B,Jian D.Serine214 of Ras2p plays a role in the feedback regulation of the Ras-cAMP pathway in the yeast Saccharomyces cerevisiae.FEBS Lett. 2010;584:2333-8
23.
Wang Y,Xu X,Riggs M,Rodgers L,Wigler M.byr2, a Schizosaccharomyces pombe gene encoding a protein kinase capable of partial suppression of the ras1 mutant phenotype.Mol Cell Biol. 1991;11:3554-63
24.
Gronwald W,Huber F,Grunewald P, et al.Solution structure of the Ras binding domain of the protein kinase Byr2 from Schizosaccharomyces pombe.Structure. 2001;9:1029-41
25.
Fukui Y,Yamamoto M.Isolation and characterization of Schizosaccharomyces pombe mutants phenotypically similar to ras1.Mol Gen Genet. 1988;215:26-31
26.
Chang EC,Barr M,Wang Y,Jung V,Xu HP,Wigler MH.Cooperative interaction of S. pombe proteins required for mating and morphogenesis.Cell. 1994;79:131-41
27.
Verde F,Wiley DJ,Nurse P.Fission yeast orb6, a ser/thr protein kinase related to mammalian rho kinase and myotonic dystrophy kinase, is required for maintenance of cell polarity and coordinates cell morphogenesis with the cell cycle.Proc Natl Acad Sci U S A. 1998;95:7526-31
28.
Wheatly E,Rittinger K.Interaction between Cdc42 and the scaffold protein Scd2: requirement of SH3 domains for GTPase binding.Biochem J. 2005;388:177-84
29.
Li YC,Chen CR,Chang EC.Fission yeast Ras1 effector Scd1 interacts with the spindle and affects its proper formation.Genetics. 2000;156:995-1004
30.
Li Y,Chang EC.Schizosaccharomyces pombe Ras1 effector, Scd1, interacts with Klp5 and Klp6 kinesins to mediate cytokinesis.Genetics. 2003;165:477-88
31.
Tanaka K,Nakafuku M,Satoh T, et al.S. cerevisiae genes IRA1 and IRA2 encode proteins that may be functionally equivalent to mammalian ras GTPase activating protein.Cell. 1990;60:803-7
32.
Tanaka K,Lin BK,Wood DR,Tamanoi F.IRA2, an upstream negative regulator of RAS in yeast, is a RAS GTPase-activating protein.Proc Natl Acad Sci U S A. 1991;88:468-72
33.
Xu G,Lin B,Tanaka K, et al.The catalytic domain of the neurofibromatosis type 1 gene product stimulates ras GTPase and complements ira mutants of S. cerevisiae.Cell. 1990;63:835-41
34.
Harashima T,Anderson S,Yates JR,Heitman J.The kelch proteins Gpb1 and Gpb2 inhibit Ras activity via association with the yeast RasGAP neurofibromin homologs Ira1 and Ira2.Mol Cell. 2006;22:819-30
35.
Peeters R,Louwet W,Gelade R, et al.Kelch-repeat proteins interacting with the Gα protein Gpa2 bypass adenylate cyclase for direct regulation of protein kinase A in yeast.Proc Natl Acad Sci U S A. 2006;103:13034-9
36.
Phan VT,Ding VW,Li F,Chalkley RJ,Burlingame A,McCormick F.The RasGAP proteins Ira2 and neurofibromin are negatively regulated by Gpb1 in yeast and ETEA in humans.Mol Cell Biol. 2010;30:2264-79
37.
Mitts MR,Bradshaw-Rouse J,Heideman W.Interactions between adenylate cyclase and the yeast GTPase-activating protein IRA1.Mol Cell Biol. 1991;11:4591-8
38.
Broek D,Toda T,Michell T, et al.The S. cerevisiae CDC25 gene product regulates the RAS/Adenylate cyclase pathway.Cell. 1987;48:789-99
39.
Haney S,Broach JR.Cdc25p, the guanine nucleotide exchange factor for the Ras proteins of Saccharomyces cerevisiae, promotes exchange by stabilizing Ras in a nucleotide-free state.J Biol Chem. 1994;269:16541-8
40.
Damak F,Boy-Marcotte E,Le-Roscouet D,Guilbaud R,Jacquet M.SDC25, a CDC25-like gene which contains a RAS-activating domain and is a dispensable gene of Saccharomyces cerevisiae.Mol Cell Biol. 1991;11:202-12
41.
Onken B,Wiener H,Philips MR,Chang EC.Compartmentalized signaling of Ras in fission yeast.Proc Natl Acad Sci U S A. 2006;103:9045-50
42.
Hughes DA,Fukui Y,Yamamoto M.Homologous activators of ras in fission yeast and budding yeast.Nature. 1990;344:355-7
43.
Tratner I,Fourticq-Esqueoute A,Tillit J,Baldacci G.Identification and characterization of the S. pombe gene efc25+, a new putative guanine nucleotide exchange factor.Gene. 1997;193:203-10
44.
Papadaki P,Pizon V,Onken B,Chang EC.Two ras pathways in fission yeast are differentially regulated by two ras guanine nucleotide exchange factors.Mol Cell Biol. 2002;22:4598-606
45.
Goodman LE,Judd SR,Farnsworth CC, et al.Mutants of Saccharomyces cerevisiae defective in the farnesylation of Ras proteins.Proc Natl Acad Sci U S A. 1990;87:9665-9
46.
He B,Chen P,Chen SY,Vancura KL,Michaelis S,Powers S.RAM2, and essential gene of yeast, and RAM1, encode the two polypeptide components of the farnesyltransferase that prenylates a-factor and Ras proteins.Proc Natl Acad Sci U S A. 1991;88:11373-7
47.
Finegold AA,Johnson DI,Farnsworth CC, et al.Protein geranylgeranyltransferase of Saccharomyces cerevisiae is specific for Cys-Xaa-Xaa-Leu motif proteins are requires the CDC43 gene product but not the DPR1 gene product.Proc Natl Acad Sci U S A. 1991;88:4448-52
48.
Finegold AA,Schafer WR,Rine J,Whiteway M,Tamanoi F.Common modifications of trimeric G proteins and ras protein: involvement of polyisoprenylation.Science. 1990;249:165-9
49.
Urano J,Tabancay AP,Yang W,Tamanoi F.The Saccharomyces cerevisiae Rheb G-protein is involved in regulating canavanine resistance and arginine uptake.J Biol Chem. 2000;275:11198-206
50.
Yang W,Urano J,Tamanoi F.Protein farnesylation is critical for maintaining normal cell morphology and canavanine resistance in Schizosaccharomyces pombe.J Biol Chem. 2000;275:429-38
51.
Fujiyama A,Tamanoi F.RAS2 protein of Saccharomyces cerevisiae undergoes removal of methionine at N terminus and removal of three amino acids at C terminus.J Biol Chem. 1990;265:3362-8
52.
Fujiyama A,Tsunasawa S,Tamanoi F,Sakiyama F.S-farnesylation and methyl esterification of C-terminal domain of yeast RAS2 protein prior to fatty acid acylation.J Biol Chem. 1991;266:17926-31
53.
Stimmel JB,Deschenes RJ,Volker C,Stock J,Clarke S.Evidence for an S-farnesylcysteine methyl ester at the carboxyl terminus of the Saccharomyces cerevisiae RAS2 protein.Biochemistry. 1990;29:9651-9
54.
Boyartchuk VL,Ashby MN,Rine J.Modulation of Ras and a-factor function by carboxyl-terminal proteolysis.Science. 1997;275:1796-800
55.
Hrycyna CA,Sapperstein SK,Clarke S,Michaelis S.The Saccharomyces cerevisiae STE14 gene encodes a methyltransferase that mediates C-terminal methylation of a-factor and RAS proteins.EMBO J. 1991;10:1699-709
56.
Griggs AM,Hahne K,Hrycyna C.Functional oligomerization of the Saccharomyces cerevisiae isoprenylcysteine carboxyl methyltransferase, Ste14p.J Biol Chem. 2010;285:13380-7
57.
Imai Y,Davey J,Kawagoshi-Kobayashi M,Yamamoto M.Genes encoding farnesyl cysteine carboxyl methyltransferase in Schizosaccharomyces pombe and Xenopus laevis.Mol Cell Biol. 1997;17:1543-51
58.
Fujiyama A,Tamanoi F.Processing and fatty acid acylation of RAS1 and RAS2 proteins in Saccharomyces cerevisiae.Proc Natl Acad Sci U S A. 1986;83:1266-70
59.
Lobo S,Greentree WK,Linder ME,Deschenes RJ.Identification of a Ras palmitoyltransferase in Saccharomyces cerevisiae.J Biol Chem. 2002;277:41268-73
60.
Swarthout JT,Lobo S,Farh L, et al.DHHC9 and GCP16 constitute a human protein fatty acyltransferase with specificity for H- and N-Ras.J Biol Chem. 2005;280:31141-8
61.
Mitchell DA,Vasudevan A,Linder ME,Deschenes RJ.Protein palmitoylation by a family of DHHC protein S-acyltransferase.J Lipid Res. 2006;47:1118-27
62.
Goodman LE,Perou CM,Fujiyama A,Tamanoi F.Structure and expression of yeast DPR1, a gene essential for the processing and intracellular localization of ras proteins.Yeast. 1988;4:271-81
63.
Williamson T,Schwartz JM,Kell DB,Stateva L.Deterministic mathematical models of the cAMP pathway in Saccharomyces cerevisiae.BMC Syst Biol. 2009;3:70-84
