Abstract
Microorganisms play a crucial role in the stability and functioning of ecosystems, responsible for essential processes such as soil nutrient cycling, plant growth, marine biogeochemical cycles, and human health. The conservation of microorganisms and microbiomes has become a priority in biotechnology and ecosystem sustainability. The preservation of these organisms is crucial not only to maintain biodiversity but also to ensure they continue to fulfill their vital roles in the ecosystem. Their role in maintaining ecosystem stability is urgent and underscores the importance of their conservation. Current conservation techniques, such as cryopreservation, freeze-drying, and storage in dry media, are essential to preserve their viability, genetic stability, and functionality. However, effective conservation goes beyond merely preserving survival; it is crucial to maintain their functionality and genetic diversity intact. Emerging methods, such as the use of nanoparticles, vitrification, and biofilms, have shown great potential to improve the protection of microorganisms from extreme environmental conditions, allowing for more effective and long-term conservation. The development of new conservation technologies is vital to overcoming the limitations of traditional methods. These innovations not only improve the viability and functionality of microorganisms but also facilitate the restoration of degraded ecosystems and foster progress in fields such as medicine, agriculture, and industry. Ensuring the conservation of these organisms is critical to ensuring the health and sustainability of our ecosystems and humanity in the future.
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