Abstract
The purpose of this report is to demonstrate that selective homonuclear decoupling can be used as an alternative for 13C NMR method for identifying configuration of an isolated (mid-chain) double bond in some lipids. Using homodecoupling of allylic protons, the coupling constants Jcis or Jtrans between the olefinic protons of some fatty acid methyl esters and related compounds with (n–7) and (n–9) terminal structures were evaluated. The optimal conditions of the homodecoupling experiments on these compounds were examined. Empirical rules were proposed to predict the results of homodecoupling experiments on lipid with an isolated double bond.
