Abstract
Members of genus Xenorhabdus are symbiotically associated with entomopathogenic nematodes of genus Steinernema. Herein, we describe the isolation and purification of four novel metabolites, xenocyloins G-J (1–4), from liquid cultures of Xenorhabdus bovienii SN52 by extensive column chromatography and semi-preparative HPLC. Chemical structures of the xenocyloin derivatives were determined based on comprehensive NMR spectra and HR-ESI-MS analyses. All compounds exhibited significant collagen-induced anti-platelet aggregation activities, among which xenocyloin H (2) exhibited the strongest activity with an inhibition rate of 96.0 ± 0.1% at a concentration of 50 μM. The xenocyloin G (1) and H (2) exhibited anti-platelet activities with IC50 values which were 31.7 ± 4.4 μM and 27.5 ± 3.5 μM respectively; whilst under similar conditions the IC50 value for aspirin was 289.5 ± 15.7 μM, nearly ten times less than 1 and 2.
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