Free accessResearch articleFirst published online 2017-9
Simultaneous Quantification of a Herbal Combination of Pueraria lobata,Salvia miltiorrhiza and Panax notoginseng by Rapid Resolution Liquid Chromatography
Pueraria lobata, Salvia miltiorrhiza and Panax notoginseng are traditional Chinese medicines which have been used for thousands of years either singly or in combination. However, the quality of formulas containing either all or part of the three herbs is, at present, controlled by only one or two easily detectable compounds.. A rapid resolution liquid chromatography (RRLC) method has been developed for the quality control of the three herbs in combination through ten active ingredients originating from the raw herbs. The analysis was achieved in a run of less than 25 minutes. Using the same method, the finger-prints of the three herbs are detectable in the chromatogram, and so the method could be applied for identification of different species in the same genus. Four formula preparations {xinkeshu capsule (XKSC), xinkeshupian (XKSP), fufang-danshenpian (FFDSP), and naodeshengpian (NDSP)} were analyzed by this new RRLC method. Controlling only one or two easily detectable ingredients is far from enough to assure the quality of the formula preparations. However, controlling multiple ingredients originating from the raw herbs and their ratios is an effective and feasible approach to solve this problem.
Chinese Pharmacopoeia Commission. (2010) Pharmacopoeia of the People's Republic of China. China Medical Science and Technology Press.
2.
WongKH, LiGQ, LiKM, Razmovski-NaumovskiV, ChanK. (2011) Kudzu root: traditional uses and potential medicinal benefits in diabetes and cardiovascular diseases. Journal of Ethnopharmacology, 134, 584–607.
3.
DuG, ZhaoHY, ZhangQW, LiGH, YangFQ, WangY, LiYC, WangYT. (2010) A rapid method for simultaneous determination of 14 phenolic compounds in Radix Puerariae using microwave-assisted extraction and ultra high performance liquid chromatography coupled with diode array detection and time-of-flight mass spectrometry. Journal of Chromatography A, 1217, 705–714.
4.
WangBQ. (2010) Salvia miltiorrhiza: Chemical and pharmacological review of a medicinal plant. Journal of Medicinal Plants Research, 4, 2813–2820.
5.
YangX, XiongX, WangH, WangJ. (2014) Protective effects of Panax notoginseng saponins on cardiovascular diseases: a comprehensive overview of experimental studies. Evidence-Based Complement Alternative Medicine, 2014, 204840.
6.
PanC, HuoY, AnX, SinghG, ChenM, YangZ, PuJ, LiJ. (2012) Panax notoginseng and its components decreased hypertension via stimulation of endothelial-dependent vessel dilatation. Vascular Pharmacology, 56, 150–158.
7.
KoonCM, WooKS, LeungPC, FungKP. (2011) Salviae miltiorrhizae radix and Puerariae lobatae radix herbal formula mediates anti-atherosclerosis by modulating key atherogenic events both in vascular smooth muscle cells and endothelial cells. Journal of Ethnopharmacology, 138, 175–183.
8.
XiaW, SunC, ZhaoY, WuL. (2011) Hypolipidemic and antioxidant activities of sanchi (radix notoginseng) in rats fed with a high fat diet. Phytomedicine, 18, 516–520.
9.
DingM, ZhaoGR, YeTX, YuanYJ, GuoZX. (2006) Salvia miltiorrhiza protects endothelial cells against oxidative stress. Journal of Alternative and Complementary Medicine, 12, 5–6.
10.
WangY, WangP, XuC, YangY, LiJ, ChenT, LiZ, CuiW, ZhouQ, SunS, LiH. (2014) Macro-fingerprint analysis-through-separation of licorice based on FT-IR and 2DCOS-IR. Journal of Molecular Structure, 1070, 1–9.
11.
CheungDWS, KoonCM, WatE, KoCH, ChanJYW, YewDTW, LeungPC, ChanWY, LauCBS, FungKP. (2013) A herbal formula containing roots of Salvia miltiorrhiza (Danshen) and Pueraria lobata (Gegen) inhibits inflammatory mediators in LPS-stimulated RAW 264.7 macrophages through inhibition of nuclear factor kB (NFkB) pathway. Journal of Ethnopharmacology, 145, 776–783.
12.
KwokT, LeungPC, LamC, HoS, WongCK, ChengKF, ChookP. (2014) A randomized placebo controlled trial of an innovative herbal formula in the prevention of atherosclerosis in postmenopausal women with borderline hypercholesterolemia. Complementary Therapies in Medicine, 22, 473–480.
13.
LiuT, QinCL, ZhangY, KangLY, SunYF, ZhangBL. (2002) Effect of dan-shen, san-qi of different proportion on platelet aggregation and adhesion in normal rabbits. Zhong Guo Zhong Yao Za Zhi, 27, 609–611.
14.
The United States Pharmacopeial Convention. (2014) Herbal Medicines Compendium. The United States Pharmacopeial Convention.
15.
MaJ, MaYC, WangD, HouFF, LuoM, LuS, GoreckiDC, PatelAV. (2011) Simultaneous quantification of Panax and Epimedium species using rapid resolution liquid chromatography (RRLC). Natural Product Communications, 6, 581–586.
16.
MaYC, ZhuJ, BenkrimaL, LuoM, SunLH, SainS, KontK, Plaut-CarcassonYY. (1996) A comparative evaluation of ginsenosides in commercial Ginseng products and tissue culture samples using HPLC. Journal of Herbs, Spices & Medicinal Plants, 3, 41–50.
17.
MaYC, LuoM, MalleyL, DoucetM. (1996) Distribution and proportion of major ginsenosides and quality control of Ginseng products. Chinese Journal of Medicinal Chemistry, 6, 21+11–20.
18.
MaYC, WangXQ, HouFF, MaJ, LuoM, LuS, JinP, TerevskyN, ChenA, XuI, PatelAV, GoreckiD. (2011) Rapid resolution liquid chromatography (RRLC) analysis for quality control of Rhodiola rosea roots and commercial standardized products. Natural Product Communications, 6, 645–650.
ChenSB, LiuHP, TianRT, YangDJ, ChenSL, XuHX, ChanAS, XiePS. (2006) High-performance thin-layer chromatographic fingerprints of isoflavonoids for distinguishing between Radix Puerariae lobate and Radix Puerariae thomsonii. Journal of Chromatography A, 1121, 114–119.
21.
LiuX, ZhangJ, ChenX. (2007) Separation and determination of three water-soluble compounds in Salvia miltiorrhiza Bunge and two related traditional medicinal preparations by flow injection-capillary electrophoresis. Journal of Chromatography B, 852, 325–332.
22.
ChangQ, SunL, ZhaoRH, ChowMS, ZuoZ. (2008) Simultaneous determination of ten active components in traditional Chinese medicinal products containing both Gegen (Pueraria lobata) and Danshen (Salvia miltiorrhiza) by high-performance liquid chromatography. Phytochemical Analysis, 19, 368–375.
23.
WangQ, FengYF, RuiW, ZhongYM, GuoJ. (2014) Rapid analysis of saponins in Panax notoginseng by UPLC /Q-TOF MS. Natural Product Research & Development, 26, 1233–1239
24.
LuY, YuK, QuHB, ChengYY. (2006) Development of an HPLC–UV–ELSD method for quantification of multiple components of a Chinese medicine made from Radix Salvia miltiorrhiza and Panax notoginseng. Chromatographia, 65, 19–24.
25.
WanJB, YangFQ, LiSP, WangYT, CuiXM. (2006) Chemical characteristics for different parts of Panax notoginseng using pressurized liquid extraction and HPLC-ELSD. Journal of Pharmaceutical and Biomedical Analysis, 41, 1596–1601.
26.
MaYC, ManiA, CaiYL, ThomsonJ, MaJ, PeudruF, ChenS, LuoM, ZhangJZ, ChapmanRG, ShiZT. (2016) An effective identification and quantification method for Ginkgo biloba flavonol glycosides with targeted evaluation of adulterated products. Phytomedicine, 23, 377–387.
