Production of Schisandrin A and Schisandrin B from Callus and Suspension Cell Cultures of Schisandra chinensis

Abstract

For establishing the fermentation system of synthetic schizandrin A and schizandrin B, culture conditions of the Schisandra chinensis callus and the suspension cell were studied in this paper. The friable calluses of Schisandra chinensis (Turcz.) Baill. were induced from hypocotyls in Murashige-Skoog (MS) solidified medium supplemented with hormone 6-benzylaminopurine (6-BA) and 2,4-Dichlorophenoxyacetic butyl acetate (2,4-D) in different concentrations, and suspension cells initiated from friable callus were cultured in MS liquid medium with various concentrations and combinations of 6-BA, 2,4-D and kinetin (KT). The optimal culture condition for callus inducement was found to be MS solidified medium with 6-BA 1.0 mg/l and 2,4-D 0.3 mg/l and the optimal condition for suspension cells was MS liquid medium with 6-BA 1.0 mg/l, 2,4-D 0.2 mg/l and KT 0.5 mg/l. UPLC/Q-TOF-MS method was used for accurate identification of schisandrin A and schisandrin B in the seeds, callus and suspension cells of S. chinensis. And HPLC analytical method was used successfully for identification and quantification of these metabolites in cultures of S. chinensis. As a result, schisandrin A was obtained 0.251 mg/g, 0.118 mg/g and 0.115 mg/g from seeds, callus and suspension cells and schisandrin B was 0.142 mg/g, 0.086 mg/g and 0.05 mg/g from seeds, callus and suspension cells, respectively. Our datas indicate that callus and suspension cells had capability as seeds of S. chinensis for schisandrin A and schisandrin B synthesizing and can be used as potential sources of these biologically active lignans.