Effective Production of Aromatic Polyketides in Streptomyces using a Combined-Culture Method

Abstract

Combined-culture is a fermentation method which efficiently induces secondary metabolite production in Streptomyces by co-culturing them with mycolic acid-containing bacteria. As a result of combined-culture screening of our terrestrial Streptomyces collection using UV-HPLC, one of the tested strains, Streptomyces sp. TAKO-2, produced two known aromatic polyketides, julichrome Q₆ (1) and julichrome Q_8·8 (2), when co-cultured with the mycolic acid-containing bacterium Tsukamurella pulmonis TP-B0596. The structures of 1 and 2 were confirmed by spectroscopic analysis and literature data.

Keywords

Combined-culture Streptomyces Mycolic acid-containing bacteria Tsukamurella Secondary metabolites

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