Two new 13, 14/14, 15-disecopregnane-type skeleton C21 steroidal aglycones, neocynapanogenin G (1) and neocynapanogenin H (2), were isolated from the hydrolyzed extract of the CHC13 soluble extract of the roots of Cynanchun paniculatum. Their structures were determined on the basis of chemical evidence and extensive spectroscopic methods, including ID and 2D NMR spectroscopy. Compound 1 displayed significant inhibition of the Hedgehog signaling pathway in vitro.
BarakatMT, HumkeE-W, ScottMP., (2010) Learning from Jekyll to control Hyde: Hedgehog signaling in development and cancer. Trends in Molecular Medicine, 16, 337–348;
2.
AmakyeD, JaganiZ, DorschM. (2013) Unraveling the therapeutic potential of the Hedgehog pathway in cancer. Nature Medicine, 19, 1410–1422;
3.
JiangSP, ChengYX. (1994) Researc progress in radix Cynanchi paniculati and paeonol. China Journal of Chinese Materia Medica, 19, 311–314;
4.
MitsuhashiH, HayashiK, NomuraT. (1966) Studies on the constituents of Asclepiadaceae plants. XVIII. Components of Cynanchum paniculatum Kitagawa. Chemical and Pharmaceutical Bulletin, 14, 779–783.
5.
SugamaK, HayashiK, MitsuhashiH, KanekoK. (1986) Studies on the constituents of Asclepiadaceae plants. LXVI. The structures of three new glycosides, cynapanosides A, B, and C, from the Chinese drug 'Xu-Chang-Qing', Cynanchum paniculatum Kitagawa. Chemical and Pharmaceutical Bulletin, 34, 4500–4507;
6.
SugamaK, HayashiK.(1988) A glycoside from dried roots of Cynanchum paniculatum. Phytochemistry, 27, 3984–3986;
7.
LiSL, TanH, ShenYM, KawazoeK, and HaoXJ. (2004) A pair of new C-21 steroidal glycoside epimers from the roots of Cynanchum paniculatum.Journal of Natural Products, 67, 82–84;
8.
DouJ, LiP, BiZM, ZhouJL. (2007) New C21 steroidal glycoside from Cynanchum paniculatum.Chinese Chemical Letters, 18, 300–302;
9.
KimCS, OhJY, ChoiSU, LeeKR. (2013) Chemical constituents from the roots ofCynanchum paniculatum and their cytotoxic activity. Carbohydrate Research, 381, 1–5.
10.
ZhangL, ChenF-Y, XuSF, YeYP, LiXY., (2014) Steroidal aglycones from stems of Marsdenia tenacissima that inhibited the Hedgehog signaling pathway. Natural Product Communications, 9, 155–156;
11.
HymanJM, FirestoneAJ, HeineVM, ZhaoY, OcasioCA, HanK, SunM, RackPG, SinhaS, WuJJ, Solow-CorderoDE, JiangJ, RowitchDH, ChenJK. (2009) Small-molecule inhibitors reveal multiple strategies for Hedgehog pathway blockade. Proceedings of the National Academy of Sciences USA., 106, 14132–14137.
