A flavonoid was isolated from the fronds of Asplenium ruta-muraria and A. altajense (Aspleniaceae) collected in the Altai Mountains and adjacent area. The compound was identified as kaempferol 3-O-β-[(6″′-E-caffeoylglucopyranosyl)-(1→3)-glucopyranoside]-7-O-β-glucopyranoside (1) by UV, 1H and 13C NMR spectroscopy, LC-MS, and acid and alkaline hydrolyses. Another flavonoid (2) was isolated from A. altajense, as a minor compound, together with 1 and identified as deacylated compound 1, i.e. kaempferol 3-O-laminaribioside-7-O-glucoside. They were found in nature for the first time.
SasakiA. (2008) Aspleniaceae. In Illustrated Flora of Ferns & Fern-allies of South Pacific Islands. National Museum of Nature and Science, Japan (Ed.) Tokai University Press, Hatano. 133–146.
2.
IwatsukiK. (1995) Aspleniaceae. In Flora of Japan. Vol. I. Peridophyta and Gymnospermae. IwatsukiK, YamazakiT, BouffordDEOhbaH. (Eds.) Kodansha, Tokyo. 98–111.
3.
MalyshevLL. (Ed.) (2000) Aspleniaceae. In Flora of Siberia. Vol. 1. Lycopodiaceae – Hydrocharitaceae. Science Publishers, Enfield. 56–61.
IwashinaT, MatsumotoS, KitajimaJ, NakamuraT, KokubugataG, SuleimanM, SaidIM. (2010) Apigenin di- and trirhamnoside from Asplenium normale in Malaysia. Natural Product Communications, 5, 39–42
7.
IwashinaT, MatsumotoS. (1990) Flavonoid profiles of Asplenium normale in Nepal with taxonomic comments. In Cryptogams of the Himalayas, Vol. 2. Central and Eastern Nepal.WatanabeM, MallaSB. (Eds.) National Science Museum, Tokyo. 179–185
8.
IwashinaT, MatsumotoS. (1994) Flavonoid variation and evolution in Asplenium normale and related species (Aspleniaceae). Journal of Plant Research, 107, 275–282
9.
MatsumotoS, IwashinaT, KitajimaJ, MitsutaS. (2003) Evidence by flavonoid markers of four natural hybrids among Asplenium normale and related species (Aspleniaceae) in Japan. Biochemical Systematics and Ecology, 31, 51–58
10.
FujiwaraT, UeharaA, KitajimaJ, IwashinaT, MatsumotoS, WatanoY. (2014) Genkwanin 4′-O-glucosyl-(1→2)-rhamnoside from new chemotype of Asplenium normale in Japan. Natural Product Communications, 9, 1289–1290.
11.
IwashinaT, MatsumotoS, NishidaM, NakaikeT. (1995) New and rare flavonol glycosides from Asplenium trichomanes-ramosum as stable chemotaxonomic markers. Biochemical Systematics and Ecology, 23, 283–290.
12.
IwashinaT, MatsumotoS, NishidaM, NakaikeT. (1993) The flavonoids from three Pakistani Asplenium subgenera Ceterach and Cheterachopsis species and their chemotaxonomic consideration. In Cryptogamic Flora of Pakistan, Vol. 2. NakaikeT, MalikS. (Eds.) National Science Museum, Tokyo. 301–315.
13.
IwashinaT, López-SáezJAHerreroA, KitajimaJ, MatsumotoS. (2000) Flavonol glycosides from Asplenium foreziense and its five related taxa and A. incisum. Biochemical Systematics and Ecology, 28, 665–671.
14.
IwashinaT, MatsumotoS. (2011) Flavonoid properties of six Asplenium species in Vanuatu and New Caledonia, and distribution of flavonoid and related compounds in Asplenium. Bulletin of the National Museum of Nature and Science, SeriesB, 37, 133–145.
15.
FanP, ZhaoL, HostettmanK, LouH. (2012) Chemical constituents of Asplenium ruta-muraria L. Natural Product Research, 26, 1413–1418.
16.
MabryTJMarkhamKRThomasMB. (1970) The Systematic Identification of Flavonoids. Springer, Berlin.
17.
LiN, LiX, MengD, GuoY, WangJ. (2006) Flavonoids from Camptosorus sibiricus Rupr. Journal of Asian Natural Product Research, 8, 167–171.
18.
MizunoM, KyotaniY, IinumaM, TanakaT, KojimaH, IwatsukiK. (1990) Kaempferol glycosides in Asplenium scolopendrium Newm. Zeitschrift für Naturforshung, 45c, 143–146
19.
EbiharaA. (2011) RbcL phyogeny of Japanese pteridophyte flora and implications on infrafamilial systematics. Bulletin of the National Museum of Nature and Science, Series B, 37, 63–74.
