Two new (3, 4) and two known phenolic derivatives (1, 2) were isolated from Radix Astragali. The structures of 1–4 were elucidated by extensive spectroscopic analysis. The anti-inflammatory activities of the isolated compounds were evaluated in LPS-induced mouse peritoneal macrophages. All four compounds exhibited potent inhibitory effects on TNF-α production and TNF-α, COX-2, IL-1β, IL-6 and iNOS mRNA expression at 50 μM.
MaX.Q., ShiQ., DuanJ.A., DongT.T.X., TsimK.W.K. (2002) Chemical analysis of Radix Astragali (Huangqi) in China: A comparison with its adulterants and seasonal variations. Journal of Agriculture and Food Chemistry, 50, 4861–4866.
2.
HanQ.B., SongJ.Z., QiaoC.F., WongL., XuH.X. (2007) Preparative isolation of cyclolanostane-type saponins from Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao by TLC-MS/MS guided high-speed counter-current chromatography. Journal of Separation Science, 30, 135–140.
3.
LeeY.S., HanO.K., ParkC.W., YangC.H., JeonT.W., YoW.K., KimS.H., KimH.J. (2005) Pro-inflammatory cytokine gene expression and nitric oxide regulation of aqueous extracted Astragali radix in RAW 264.7 macrophage cells. Journal of Ethnopharmacology, 100, 289–294.
4.
SuoM.R., YangJ.S., ZhangZ.S. (2008) Two new compounds from the stem of Vernonia cumingiana. Chinese Chemical Letters, 19, 180–182.
5.
CollinJ.P., JouvenotD., KoizumiM., SauvageJ.P. (2005) Light-driven expulsion of the sterically hindering ligand L in tris-diimine ruthenium(II) complexes of the ru(phen)2(L)2+ family: A pronounced ring effect. Inorganic Chemistry, 44, 4693–4698.
6.
SunW.C., MooreJ.N., HurleyD.J., Van den plasM.L., LindenJ., CaoZ., MurrayT.F. (2008) Adenosine A2A receptor agonists inhibit lipopolysaccharide-induced production of tumor necrosis factor α by equine monocytes. Veterinary Immunology and Immunopathology, 121, 91–100.