One new schiartane-type triterpene, heteroclitalactone N (1), and four known compounds (2–5), seco-coccinic acid F, dihydroguaiaretic acid, schizanrin F, and kadsuralignan B were isolated from the stems of Kadsura heteroclita. Their structures were determined by the combination of spectroscopic and chemical methods, including 1D-, 2D-NMR, and CD spectra as well as by comparing with the NMR data reported in the literature. The cytotoxic activities of all isolated compounds were evaluated on three human cancer cell lines. Compound 3 exhibited moderate to weak cytotoxic activities on three human cancer cell lines, OVCAR, HT-29, and A-549, with IC50 values ranging from 16.2 to 36.4 μM.
(a) ChenD.F., XuG.J., YangX.W., HattoriM., TezukaY., KikuchiT., NambaT. (1992) Dibenzocyclo-octadiene lignans from Kadsura heteroclita. Phytochemistry, 31, 629–632; (b) Yang XW, Miyashiro H, Hattori M, Namba T, Tezuka Y, Kikuchi T, Chen DF, Xu GJ, Hori T, Extine M, Mizuno H. (1992) Isolation of novel lignans, heteroclitins F and G, from the stems of Kadsura heteroclita, and anti-lipid peroxidative actions of heteroclitins A-G and related compounds in the in vitro rat liver homogenate system. Chemical and Pharmaceutical Bulletin, 40, 1510–1516.
2.
(a) WangW., LiuJ., HanJ., XuZ., LiuR., LiuP., WangW., MaX., GuanS., GuoD. (2006) New triterpenoids from Kadsura heteroclita and their cytotoxic activity. Planta Medica, 72, 450–457; (b) Xu LJ, Peng ZG, Chen HS, Wang J, Xiao PG. (2010) Chemistry & Biodiversity, 7, 2289–2295; (c) Pu JX, Yang LM, Xiao WL, Li RT, Lei C, Gao XM, Huang SX, Li SH, Zheng YT, Huang H, Sun HD. (2008). Phytochemistry, 69, 1266–1272.
3.
XiaoW.L., LiR.T., HuangS.X., PuJ.X., SunH.D. (2008) Triterpenoids from the Schisandraceae family. Natural Product Reports, 25, 871–891.
4.
(a) LiR.T., HanQ.B., ZhengY.T., WangR.R., YangL.M., LuY., SangS.Q., ZhengQ.T., ZhaoQ.S., SunH.D. (2005) Structure and anti-HIV activity of micrandilactones B and C, new nortriterpenoids possessing a unique skeleton from Schisandra micrantha. Chemical Communications, 2936–2938; (b) Lei C, Huang SX, Chen JJ, Yang LB, Xiao WL, Chang Y, Lu Y, Huang H, Pu JX, Sun HD. (2008) Journal of Natural Products, 71, 1228–1232.
5.
(a) WangN., LiZ., SongD., LiW., FuH., KoikeK., PeiY., JingY., HuaH. (2008) Lanostane-type triterpenoids from the roots of Kadsura coccinea. Journal of Natural Products, 71, 990–994; (b) Filleur F, Le Bail JC, Duroux JL, Simon A, Chulia AJ. (2001) Planta Medica, 67, 700–704; (c) Wu MD, Huang RL, Kuo LMY, Hung CC, Ong CW, Kuo YH. (2003) Chemical & Pharmaceutical Bulletin, 51, 1233–1236; (d) Li HR, Feng YL, Yang ZG, Wang J, Daikonya A, Kitanaka S, Xu LZ, Yang SL. (2006) Chemical & Pharmaceutical Bulletin, 54, 1022–1025.
6.
AlleyM.C., ScudieroD.A., MonksA., HurseyM.L., CzerwinskiM.J., FineD.L., AbbottB.J., MayoJ.G., ShoemakerR.H., BoydM.R. (1988) Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay. Cancer Research, 48, 589–601.
