A callus culture of Iris pseudacorus L. (Iridaceae) was established from plant leaves using a modified Murashige and Skoog medium. A derivative of cinnamic acid (lavandoside) (1), a neolignan (dehydrodiconiferyl alcohol-4-O-β-D-glucopyranoside) (2) as well as three isoflavonoids, tectoridin (3), tectorigenin (4), and iristectorigenin A (5) were isolated from the callus culture. Under normal conditions, the calli accumulated 0.4% DW of polyphenols. The addition of phenylalanine to a concentration of 1 mM resulted in a 1.5-fold increase in isoflavonoid production, allowing the accumulation of 0.69% of polyphenols in the callus dry weight. Tectorigenin, a promising chemotherapeutic and chemopreventive agent for the treatment of carcinomas, was produced in I. pseudacorus calli in high quantities (0.3% DW).
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2.
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3.
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4.
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5.
JiangC.P., DingH., ShiD.H., WangY.R., LiE.G., WuJ.H. (2012) Pro-apoptotic effects of tectorigenin on human hepatocellular carcinoma HepG2 cells. World Journal of Gastroenterology, 18, 1753–1764; (b) Mischenko NP, Fedoreyev SA, Glazunov VP, Tchernoded GK, Bulgakov VP, Zhuravlev YN. (1999) Anthraquinone production by callus cultures of Rubia cordifolia. Fitoterapia, 70, 552–557.
