Essential oils (EO, 15 collective samples and 47 individual samples) of Thymbra capitata collected from Moroccan Western Rif were analyzed using gas chromatography (GC) in combination with retention indices (RI), gas chromatography-mass spectrometry (GC-SM) and 13C NMR spectroscopy. Twenty components were identified. Carvacrol (68.2%–85.9 %) was by far the major component of all the samples, while the content of thymol (0.1-0.3%) was very low. Other components present in appreciable amounts were γ-terpinene (up to 8.9%), p-cymene (up to 7.1%), linalool (up to 4.4%) and (E)-β-caryophyllene (up to 4.1%). In contrast, the yield of EO varied drastically from sample to sample (0.5–3.7%). No correlation could be established between yield of EO and altitude, pH, chemical composition and granularity of the soil. Cultivation under controlled conditions is suggested to improve the quantitative characteristics of carvacrol-rich Moroccan T. capitata.
ZarzueloA., CrespoE. (2002) The medicinal and non–medicinal uses of Thyme. In Medicinal and Aromatic Plants–Industrial Profiles. Vol. 17. Thyme. The genus Thymus. Stahl-BishupE., SáezF. (Eds), Taylor & Francis, London, UK.263–292.
2.
VilaR., (2002) Flavonoids and further polyphenols in the genus Thymus. In Medicinal and Aromatic Plants–Industrial Profiles. Vol. 17. Thyme. The genus Thymus. Stahl-BishupE., SáezF. (Eds), Taylor & Francis, London, UK.144–176.
3.
FigueiredoA.C., BarrosoJ.G., PedroL.G., SalgueiroL., MiguelM.G., FaleiroM.L. (2008) Portuguese Thymbra and Thymus species volatiles: chemical composition and biological activities. Current Pharmaceutical Design, 14, 3120–3140.
4.
BounatirouS., SmitiS., MiguelM.G., FaleiroL., RejebM.N., NeffatiM., CostaM.M., FigueiredoA.C., BarrosoJ.G., PedroI.G. (2007) Chemical composition, antioxidant and antibacterial activities of the essential oils isolated from Tunisian Thymus capitatus Hoff. & Link. Food Chemistry, 105, 146–155.
5.
BenjllaliB., Tantaoui-ElarakiA., Ismaili-AlaouiM., AyadiA. (1986) Méthode d'étude des propriétés antiseptiques des huiles essentielles par contact direct en milieu gélosé. Plantes Médicinales et Phytothérapie, 20, 156–167.
6.
El AjjouriM., SatraniB., GhanmiM., AafiA., FarahA., RahoutiM., AmartiF., AberchaneM. (2008) Activité antifongique des huiles essentielles de Thymus bleicherianus Pomel et Thymus capitatus (L.) Hoffm. & Link contre les champignons de pourriture du bois d'Œuvre. Biotechnologie, Agronomie, Société, Environnement, 12, 345–351.
7.
AmartiF., SatraniB., AafiA., GhanmiM., FarahA., AberchaneM., El AjjouriM., El AntryS., ChaouchA. (2008) Composition chimique et activité antimicrobienne des huiles essentielles de Thymus capitatus et de Thymus bleicherianus du Maroc. Phytothérapie, 6, 342–347.
8.
El OuariachiE.M., PaoliniJ., BouyanzerA., TomiP., HammoutiB., SalghiR., MajidiL., CostaJ. (2011) Chemical composition and antioxidant activity of essential oils and solvent extracts of Thymus capitatus (L.) Hoff. and Link from Morocco. Journal of Medicinal Plants Research, 5, 5773–5778.
9.
KuštrakD., MartinisZ., KuftinecJ., Blaževic’N. (1990) Composition of the essential oils of some Thymus and Thymbra species. Flavour and Fragrance Journal, 5, 227–231.
10.
SalgueiroL.R., PintoE., GonçalvesM.J., Pina-VazC., CavaleiroC., RodriguesA.G., PalmeiraA., TavaresC., Costa-de-OliveiraS., Martinez-de-OliveiraJ. (2004) Chemical composition and antifungal activity of the essential oil of Thymbra capitata. Planta Medica, 70, 572–575.
11.
EconomouG., PanagopoulosG., TarantilisP., KalivasD., KotoulasV., TravlosI.S., PolysiouM., KaramanosA. (2011) Variability in essential oil content and composition of Origanum hirtum L., Origanum onites L., Coridothymus capitatus (L.) and Satureja thymbra L. populations from the Greek island Ikaria. Industrial Crops and Products, 33, 236–6241.
12.
UsaiM., FoddaiM., SechiB., JulianoC., MarchettiM. (2010) Comparison of antibacterial activity of natural and hydroformylated essential oil of Thymuscapitatus growing wild in north Sardinia with commercial Thymus essential oils. Natural Product Communications, 5, 1985–1989.
13.
NapoliE.M., CurcurutoG., GiuseppeR. (2010) Screening of the essential oil composition of wild Sicilian thyme. Biochemical Systematics and Ecology, 38, 816–822.
14.
Ben El Hadj AliI., GuetatA., BoussaidM. (2012) Variation of volatiles in Tunisian populations of Thymbra capitata (L.) Cav. (Lamiaceae). Chemistry and Biodiversity, 9, 1272–1285.
Ben El Hadj AliI., GuetatA., BoussaidM. (2013) A combined approach using allozymes and volatiles for the characterization of Tunisian Thymbra capitata (L.) Cav. (Lamiaceae). Industrial Crops and Products, 43, 477–483.
17.
FleisherZ., FleisherA., (2002) Volatiles of Coridothymus capitatus chemotypes growing in Israel: aromatic plants of the Holy Land and the Sinai. XV. Journal of Essential Oil Research, 14, 105–106.
18.
KarousouR., KoureasD.N., KokkiniS. (2005) Essential oil composition is related to the natural habitats: Coridothymus capitatus and Satureja thymbra in NATURA 2000 sites of Crete. Phytochemistry, 66, 2668–2673.
19.
MiceliA., NegroC., TommasiL. (2006) Essential oil variability in Thymbra capitata (L.) Cav. growing wild in Southern Apulia (Italy). Biochemical Systematics and Ecology, 34, 528–535.
20.
TommasiL., NegroC., CerfedaA., NutricatiE., ZuccarelloV., De BellisL., MiceliA. (2007) Influence of environmental factors on essential oil variability in Thymbra capitata (L.) Cav. growing wild in southern Puglia (Italy). Journal of Essential Oil Research, 19, 572–580.
21.
FleisherA., FleisherZ., Abu-RukunS. (1984) Chemovarieties of Coridothymus capitatus L. Rchb. growing in Israel. Journal of Science Food Agriculture, 35, 495–499.
22.
Can BaserK.H. (2008) Biological and pharmacological activities of carvacrol and carvacrol bearing essential oils. Current Pharmaceutical Design, 14, 3106–3119.
23.
(a) US National Institute of Standards and Technology. (1999) PC Version 1.7 of the NIST/EPA/NIH Mass Spectra Library, Norwalk, CT; (b) König WA, Hochmuth DH, Joulain D. (2001) Terpenoids and related constituents of essential oils, Library of Massfinder 2.1. Univ. Hamburg.
24.
(a) JoulainD., KönigW.A. (1998) The atlas of spectral data of sesquiterpene hydrocarbons, E B-Verlag, Hamburg; (b) Adams RP. (2007) Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometry. 4th ed. Allured Publ. Corp., Carol Stream, IL.
25.
(a) TomiF., CasanovaJ. (2006) 13C-NMR as a tool for identification of individual components of essential oils from Labiatae. Acta Horticulturae, 723, 185–192;(b) Duquesnoy E, Castola V, Casanova J. (2007) Identification and quantitative determination of triterpenes in the hexane extract of Olea europaea L. leaves using 13C NMR spectroscopy. Phytochemical Analysis, 18, 347–353.
