Oregano (Thymbra capitata and Origanum vulgare) essential oils (EOs), cultivated and extracted in the South-East of Spain, were analysed by GC/MS to determine their composition. (E)-β-Caryophyllene (0.5–4.9%), thymol (0.2–5.8%), p-cymene (3.8–8.2%), γ-terpinene (2.1–10.7%) and carvacrol (58.7–77.4%) were determined as the main molecules. This characterisation was completed with enantioselective gas chromatography, where (-)-(E)-β-caryophyllene, (+)-α-pinene and (+)-β-pinene were determined as the main enantiomers. Antioxidant activity was evaluated positively by several methods, accounting for activity against free radicals and reducing power. Important inhibitory activity on lipoxygenase (LOX) and acetylcholinesterase (AChE) was observed supporting potential anti-inflammatory, anti-Alzheimer and insecticidal activities, mainly due to carvacrol. These properties support the potential use of oregano EOs as natural cosmetic and natural pharmaceutical ingredients.
EconomouG, PanagopoulosG, TarantilisP, KalivasD, KotoulasV, TravlosIS, PolysiouM, KaramanosA. (2011) Variability in essential oil content and composition of Origanum hirtum L., Origanum onites L., Coridothymus capitatus (L.) and Satureja thymbra L. populations from the Greek island Ikaria. Industrial Crops and Products, 33, 236–241.
2.
FariasG, BruttiO, GrauR, Di Leo LiraP, RettaD, van BarenC, VentoS, BandoniAL. (2010) Morphological, yielding and quality descriptors of four clones of Origanum spp. (Lamiaceae) from the Argentine Littoral region Germplasm bank. Industrial Crops and Products, 32, 472–480.
3.
BishtD, ChanotiyaCS, RanaM, SemwalM. (2009) Variability in essential oil and bioactive chiral monoterpenoid compositions of Indian oregano (Origanum vulgare L.) populations from northwestern Himalaya and their chemotaxonomy. Industrial Crops and Products, 30, 422–426.
4.
TateoF, MariottiM, BononiM. (1998) Essential oil composition and enantiomeric distribution of some monoterpenoid components of Coridothymus capitatus (L.) Rchb. grown on the island of Kos (Greece). Journal of Essential Oil Research, 10, 241–244.
5.
SmelcerovicA, DjordjevicA, LazarevicJ, StojanovicG. (2013) Recent advances in analysis of essential oils. Current Analytical Chemistry, 9, 61–70.
6.
DawidowiczAL, OlszowyM. (2014) Does antioxidant properties of the main component of essential oil reflect its antioxidant properties? The comparison of antioxidant properties of essential oils and their main components. Natural Product Research, 28, 1952–1963.
7.
AnwarY, SabirJSM, QureshiMI, SainiKS. (2014) 5-Lipoxygenase: A promising drug target against inflammatory diseases-biochemical and pharmacological regulation. Current Drug Targets, 15, 410–422.
8.
PundirCS, ChauhanN. (2012) Acetylcholinesterase inhibition-based biosensors for pesticide determination: A review. Analytical Biochemistry, 429, 19–31.
9.
MurrayAP, FaraoniMB, CastroMJ, AlzaNP, CavallaroV. (2013) Natural AChE inhibitors from plants and their contribution to Alzheimer's disease therapy. Current Neuropharmacology, 11, 388.
10.
Council-of-Europe. (2011) European Pharmacopoeia. European-Directorate, Strasbourg, France.
11.
NapoliEM, CurcurutoG, RubertoG. (2010) Screening of the essential oil composition of wild Sicilian thyme. Biochemical Systematics and Ecology, 38, 816–822.
12.
RussoM, SuraciF, PostorinoS, SerraD, RoccotelliA, AgosteoGE. (2013) Essential oil chemical composition and antifungal effects on Sclerotium cepivorum of Thymus capitatus wild populations from Calabria, southern Italy. Revista Brasileira De Farmacognosia-Brazilian Journal of Pharmacognosy, 23, 239–248.
13.
Palmeira-de-OliveiraA, GasparC, Palmeira-de-OliveiraR, Silva-DiasA, SalgueiroL, CavaleiroC, Pina-VazC, Martinez-de-OliveiraJ, QueirozJA, RodriguesAG. (2012) The anti-Candida activity of Thymbra capitata essential oil: Effect upon pre-formed biofilm. Journal of Ethnopharmacology, 140, 379–383.
14.
BakhyK, BenlhabibO, Al FaizC, BighelliA, CasanovaJ, TomiF. (2013) Wild Thymbra capitata from Western Rif (Morocco): essential oil composition, chemical homogeneity and yield variability. Natural Product Communications, 8, 1155–1158.
15.
Ballester-CostaC, SendraE, Fernandez-LopezJ, Perez-AlvarezJA, Viuda-MartosM. (2013) Chemical composition and in vitro antibacterial properties of essential oils of four Thymus species from organic growth. Industrial Crops and Products, 50, 304–311.
16.
AmriI, HamrouniL, HananaM, JamoussiB, LebdiK. (2014) Essential oils as biological alternatives to protect date palm (Phoenix dactylifera L.) against Ectomyelois ceratoniae Zeller (Lepidoptera: Pyralidae). Chilean Journal of Agricultural Research, 74, 273–279.
17.
HosniK, HassenI, ChaabaneH, JemliM, DallaliS, SebeiH, CasabiancaH. (2013) Enzyme-assisted extraction of essential oils from thyme (Thymus capitatus L.) and rosemary (Rosmarinus officinalis L.): Impact on yield, chemical composition and antimicrobial activity. Industrial Crops and Products, 47, 291–299.
18.
SkoulaM, GrayerRJ. (2005) Volatile oils of Coridothymus capitatus, Satureja thymbra, Satureja spinosa and Thymbra calostachya (Lamiaceae) from Crete. Flavour and Fragrance Journal, 20, 573–576.
19.
TabtiL, DibMEA, DjabouN, BenyellesNG, PaoliniJ, CostaJ, MuselliA. (2014) Control of fungal pathogens of Citrus sinensis L. by essential oil and hydrosol of Thymus capitatus L. Journal of Applied Botany and Food Quality, 87, 279–285.
20.
AliIBEH, GuetatA, BoussaidM. (2013) A combined approach using allozymes and volatiles for the characterization of Tunisian Thymbra capitata (L.) Cav. (Lamiaceae). Industrial Crops and Products, 43, 477–483.
21.
KoldasS, DemirtasI, OzenT, DemirciMA, BehcetL. (2015) Phytochemical screening, anticancer and antioxidant activities of Origanum vulgare L. ssp viride (Boiss.) Hayek, a plant of traditional usage. Journal of the Science of Food and Agriculture, 95, 786–798.
22.
VeresK, VargaE, SchelzZ, MolnarJ, BernathJ, MathéI. (2007) Chemical composition and antimicrobial activities of essential oils of four lines of Origanum vulgare subsp hirtum (Link) Ietswaart grown in Hungary. Natural Product Communications, 2, 1155–1158.
23.
StoilovaI, BailS, BuchbauerG, KrastanovA, StoyanovaA, SchmidtE, JirovetzL. (2008) Chemical composition, olfactory evaluation and antioxidant effects of an essential oil of Origanum vulgare L. from Bosnia. Natural Product Communications, 3, 1043–1046.
24.
LukasB, SchmidererC, MittereggerU, FranzC, NovakJ. (2008) Essential oil compounds of Origanum vulgare L.(Lamiaceae) from Corsica. Molecular and phytochemical analyses of the genus Origanum L. (Lamiaceae), 57, 63–70.
25.
LukasB, SchmidererC, NovakJ. (2013) Phytochemical diversity of Origanum vulgare L. subsp vulgare (Lamiaceae) from Austria. Biochemical Systematics and Ecology, 50, 106–113.
26.
BonfantiC, IanniR, MazzagliaA, LanzaCM, NapoliEM, RubertoG. (2012) Emerging cultivation of oregano in Sicily: Sensory evaluation of plants and chemical composition of essential oils. Industrial Crops and Products, 35, 160–165.
27.
Hernandez-HernandezE, Regalado-GonzalezC, Vazquez-LandaverdeP, Guerrero-LegarretaI, Garcia-AlmendarezBE. (2014) Microencapsulation, chemical characterization, and antimicrobial activity of Mexican (Lippia graveolens HBK) and European (Origanum vulgare L.) oregano essential oils. Scientific World Journal, 2014, 1–12.
28.
de FalcoE, RoscignoG, IodiceC, SenatoreF. (2013) Phytomorphological and essential-oil characterization in situ and ex situ of wild biotypes of oregano collected in the Campania region (Southern Italy). Chemistry & Biodiversity, 10, 2078–2090.
29.
MalletACT, CardosoMG, SouzaPE, MachadoSMF, AndradeMA, NelsonDL, PiccoliRH, PereiraCG. (2014) Chemical characterization of the Allium sativum and Origanum vulgare essential oils and their inhibition effect on the growth of some food pathogens. Revista Brasileira de Plantas Medicinais, 16, 804–811.
30.
MecherguiK, CoelhoJA, SerraMC, LamineSB, BoukhchinaS, KhoujaML. (2010) Essential oils of Origanum vulgare L. subsp glandulosum (Desf.) Letswaart from Tunisia: chemical composition and antioxidant activity. Journal of the Science of Food and Agriculture, 90, 1745–1749.
31.
BozinB, Mimica-DukicN, SiminN, AnackovG. (2006) Characterization of the volatile composition of essential oils of some Lamiaceae spices and the antimicrobial and antioxidant activities of the entire oils. Journal of Agricultural and Food Chemistry, 54, 1822–1828.
32.
OsmanAM, WongKKY, FernyhoughA. (2006) ABTS radical-driven oxidation of polyphenols: Isolation and structural elucidation of covalent adducts. Biochemical and Biophysical Research Communications, 346, 321–329.
33.
RubertoG, BarattaMT. (2000) Antioxidant activity of selected essential oil components in two lipid model systems. Food Chemistry, 69, 167–174.
34.
NabaviSM, MarcheseA, IzadiM, CurtiV, DagliaM, NabaviSF. (2015) Plants belonging to the genus Thymus as antibacterial agents: From farm to pharmacy. Food Chemistry, 173, 339–347.
35.
DžamićAM, NikolićBJ, GiweliAA, Mitić-ĆulafićDS, SokovićMD, RistićMS, Knežević-VukčevićJB, MarinPD. (2015) Lybian Thymus capitatus essential oil: antioxidant, antimicrobial, cytotoxic and colon pathogen adhesion-inhibition properties. Journal of Applied Microbiology in press.
36.
SaoudI, HamrouniL, GargouriS, AmriI, HananaM, FezzaniT, BouzidS, JamoussiB. (2013) Chemical composition, weed killer and antifungal activities of Tunisian thyme (Thymus capitatus Hoff. et Link.) essential oils. Acta Alimentaria, 42, 417–427.
37.
Hortigón-VinagreMP, BlancoJ, RuizT, HenaoF. (2014) Thymbra capitata essential oil prevents cell death induced by 4-hydroxy-2-nonenal in neonatal rat cardiac myocytes. Planta Medica, 80, 1284–1290.
38.
OkaY, NacarS, PutievskyE, RavidU, YanivZ, SpiegelY. (2000) Nematicidal activity of essential oils and their components against the root-knot nematode. Phytopathology, 90, 710–715.
39.
Rivas-MartínezS. (1987) Nociones sobre fitosociología, biogeografía y bioclimatología en la vegetación de España [Notions about phytosociology biogeography and bioclimatology in vegetation of Spain], University of Alcalá, Madrid.
40.
IUPAC (Ed.), (1997) Compendium of chemical terminology (the “Gold book”), Blackwell Scientific Publications, Oxford.
41.
OuBX, Hampsch-WoodillM, PriorRL. (2001) Development and validation of an improved oxygen radical absorbance capacity assay using fluorescein as the fluorescent probe. Journal of Agricultural and Food Chemistry, 49, 4619–4626.
BrandwilliamsW, CuvelierME, BersetC. (1995) Use of a free-radical method to evaluate antioxidant activity. Food Science and Technology- Lebensmittel-Wissenschaft & Technologie, 28, 25–30.
44.
OyaizuM. (1986) Studies on products of browning reaction - antioxidative activities of products of browning reaction prepared from glucosamine. Eiyogaku Zasshi= Japanese Journal of Nutrition, 44, 307–315.
45.
DormanHJD, DeansSG, NobleRC, SuraiP. (1995) Evaluation in vitro of plant essential oils as natural antioxidants. Journal of Essential Oil Research, 7, 645–651.
46.
ChristopherJ, PistoriusE, AxelrodB. (1970) Isolation of an isozyme of soybean lipoxygenase. Biochimica et Biophysica Acta, 198, 12–19.
47.
EllmanGL, CourtneyKD, AndresVjr, FeatherstoneRM. (1961) A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical Pharmacology, 7, 88–95.
48.
SokalRR, RohlfFJ. (2012) Biometry.Freeman & Co, New York.
