Seven isoflavonoids, including a new glycoside, (6aR,11aR)-medicarpin-3-O-gentiobioside (6), were isolated from the roots of Maackia amurensis using repeated column chromatography on a Toyopearl HW-50F sorbent and identified by HPLC–PDA–MS, 1H NMR, 13C, 1H–1H COSY, HSQC NMR and HMBC NMR analyses as daidzin (1), genistein-7-O-gentiobioside (2), pseudobaptigenin-7-O-gentiobioside (3), formononetin-7-O-gentiobioside (4), (6aR,11aR)-maackiain-3-O-gentiobioside (5), and 5-O-methylgenistein-7-O-gentiobioside (7). In the model of oxidative stress induced by formalin injection, the isolated isoflavone and pterocarpan glucosides 1-7 were shown to reduce the formation of malondialdehyde (MDA) and other thiobarbituric acid reactive substances (TBARS), as well as glutathione peroxidase (GPO) activity in rats.
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SaratikovAS, ChuchalinVS, Rat'kinAV, Rat'kinEV, FedoreyevSA, BulgakovVP. (2005) Hepatoprotive properties of polyphenol complexes from the wood and cell culture of M. amurensis. Eksperimental'naia i klinicheskaia farmakologiia, 68, 51–54.
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PlotnikovaAM, ShulgauZT, PlotnikovaTM, AlievOI, KuleshNI, MishchenkoNP, FedoreyevSA. (2009) Antithrombogenic and antiplatelet activities of extract from Maackia amurensis wood. Bulletin of Experimental Biology and Medicine, 147, 204–207.
