The essential oils extracted from the young stems and leaves of Chamaecyparis lawsoniana(A.Murray) Parl. have been analysed by Gas Chromatography and Gas Chromatography coupled to Mass Spectrometry. A total of 66 compounds were identified representing around the 99% of the total oil. The oil was richer in monoterpenes than in sesquiterpenes. The only main component was limonene with a percentage composition of 77.7%. The rest of compounds that contribute to the fragrance had percentage composition lower that the 3.0%: p-cymen-7-ol (3.0%), myrcene (2.4%), camphor (2.1%), δ-elemene (1.6%), oplopanonyl acetate (1.6%), methyl perillate (1.3%), terpinen-4-ol (1.0%) and β-oplopenone 1.0%. The antibacterial and antifungal activity of this oil was also tested against different microorganisms. The only fungus tested, Candida albicans,was very sensitive to the treatment with an inhibition halos of 20mm. The oil was more effective with the Gram (+) than with Gram (-) bacteria. The inhibition halos were 12mm, 12-13mm and 12-13mm for Bacillus subtilis, Staphylococcus aureus and Micrococcus luteus respectively. We report new data of the antibacterial and antifungal activity of the essential oil of this species. The essential oil of C. lawsoniana could be considered as a good natural antibacterial and antifungal agent.
VidakovićM. (1991) Conifers Morphology and Variation. Grafički Zavod Hrvatske, Zagreb, Croatia.
2.
MichenerDC. (1990) “Chamaecyparis”. in Flora of North America Vol. 2. Oxford University Press. (Online at http://hua.huh.harvard.edu/FNA/).
3.
FuL, YuYF, AdamsRP, FarjonA. (1999) “Cupressaceae”. in Flora of China Vol. 4. Published by Science Press (Beijing) and Missouri Botanical Garden Press. (Online at http://hua.huh.harvard.edu/china/).
4.
Sánchez de Lorenzo-CáceresJM. (2001) Guía de plantas ornamentales. Edicionesn Mundi-Prensa. Madrid.
5.
TurnerNJ (1979) The ethnobotany of the Southern Kwakiutl Indians of British Columbia. Economic Botany, 27, 257–310.
6.
Palá-PaúlJ, Usano-AlemanyJ, GrandaE, SoriaAC. (2009) Chemical composition, antifungal and antibacterial activity of the essential oil of Chamaecyparis nootkatensis from Spain. Natural Product Communications, 4, 1007–1010;
7.
AdamsRP, ThomasP, RushforthK. (2007) The leaf essential oils of the new conifer genus, Xanthocyparis: Xanthocyparis vietnamensis and X. nootkatensis. Journal of Essential Oil Research, 19, 30–33;
8.
ChengSS, ChangHT, WuCL, ChangST. (2007) Anti-termitic activities of essential oils from coniferous trees against Coptotermes formosanus. Bioresource Technology, 98, 456–459;
9.
ChengSS, ChuaMT, ChangEH, HuangCG, ChenWJ, ChangST. (2008) Variations in insecticidal activity and chemical compositions of leaf essential oils from Cryptomeria japonica at different ages. Bioresource Technology, 100, 465–470;
10.
ChengSS, LinHY, ChangST. (2005) Chemical composition and antifungal activity of essential oils from different tissues of Japanese cedar (Cryptomeria japonica). Journal of Agricultural and Food Chemistry, 53, 614–619;
11.
CraigAM, KarchesyJJ, BlytheLL, González-HernándezMP, SwanLR. (2004) Toxicity studies on western juniper oil (Juniperus occidentalis) and port-orford-cedar oil (Chamaecyparis lawsoniana) extracts utilizing local lymph node and acute dermal irritation assays. Toxicology Letters, 154, 217–224.
12.
DebiaggiM, PaganiL, CeredaPM, LandiniP, RomeroE. (1988) Antiviral activity of Chamaecyparis lawsoniana extract: study with Herpes simplex virus type 2. Microbiologica, 11, 55–61;
13.
DietrichG, DolanMC, Peralta-CruzJ, SchmidtJ, PiesmanJ, EisenRJ, KarchesyJJ. (2006) Repellent activity of fractioned compounds from Chamaecyparis nootkatensis essential oil against nymphal Ixodes scapularis (Acari: Ixodidae). Journal of Medical Entomology, 43, 957–961;
14.
DuT, ShupeTF, HseCY. (2011) Antifungal activities of three supercritical fluid extracted cedar oils. Holzforschung, 65, 277–284;
15.
DuringerJM, SwanLR, WalkerDB, CraigAM. (2010) Acute aquatic toxicity of western juniper (Juniperus occidentalis) foliage and Port Orford cedar (Chamaecyparis lawsoniana) heartwood oils. Environmental Monitoring and Assessment, 170, 585–598.
16.
EmamiSA, MassoomiH, MoghadamMS, AsiliJ. (2009) Identification of Volatile Oil Components from Aerial Parts of Chamaecyparis lawsoniana by GC-MS and (13)C-NMR Methods. Journal Of Essential Oil Bearing Plants, 12, 661–665;
17.
GaoH, ObandaDN, ShupeTF, HseCY, RingDR. (2008) Antifungal activities of heartwood extracts of Port-Orford cedar extractives. Holzforschung, 62, 620–623.
18.
GaoH, ShupeTF, HseCY, EberhardtTL. (2006) Antioxidant activity of extracts from the bark of Chamaecyparis lawsoniana (A. Murray) Parl. Holzforschung, 60, 459–462;
HiramatsuY, MatsuiN, OhiraT, ImaiY, MiyazakiY. (2006) Effect of hinoki (Chamaecyparis obtusa) wood-wool in tatami mat on the activity of house dust mite Dermatophagoides pteronyssinus. Journal of Wood Science, 52, 353–357.
21.
JangYS, LeeCH, KimMK, KimJH, LeeSH, LeeHS. (2005) Acaricidal activity of active constituent isolated in Chamaecyparis obtusa leaves against Dermatophagoides spp. Journal of Agricultural and Food Chemistry, 53, 1934–1937;
22.
JohnsonAP, AuckenHM, CavendishS, GannerM, WaleMCJ, WarnerM, LivermoreDM, CooksonBD. (2001) Dominance of EMRSA-15 and -16 among MRSA causing nosocomial bacteraemia in the UK: analysis of isolates from the European Antimicrobial Resistance Surveillance System (EARSS). Journal of Antimicrobial Chemotherapy, 48, 143–144;
23.
JooSS, YooYM, KoSH, ChoiW, ParkMJ, KangHY, ChoiKC, ChoiIG, JeungEB. (2010) Effects of essential oil from Chamaecypris obtusa on the development of atopic dermatitis-like skin lesions and the suppression of Th cytokines. Journal of Dermatological Science, 60, 122–125;
24.
KangTH, WangEI, YunBS, ShinCS, KimSU. (2008) Chitin Synthase 2 Inhibitory Activity of O-Methyl Pisiferic Acid and 8,20-Dihydroxy-9(11),13-abietadien-12-one, Isolated from Chamaecyparis pisifera. Biological & Pharmaceutical Bulletin, 31, 755–759;
25.
KoyamaS, YamaguchiY, TanakaS, MotoyoshiyaJ. (1997) A new substance (yoshixol) with an interesting antibiotic mechanism from wood oil of Japanese traditional tree (kiso-hinoki), Chamaecyparis obtusa. General Pharmacology, 28,797-804;
26.
KuoPM, ChuFH, ChangST, HsiaoWF, WangSY. (2007) Insecticidal activity of essential oil from Chamaecyparis formosensis Matsum. Holzforschung, 61, 595–599.
27.
LeeGS, HongEJ, GwakKS, ParkMJ, ChoiKC, ChoiIG, JangJW, JeungEB. (2010) The essential oils of Chamaecyparis obtusa promote hair growth through the induction of vascular endothelial growth factor gene. Fitorerapia, 81, 17–24;
28.
ManterDK, KarchesyJJ, KelseyRG. (2006) The sporicidal activity of yellow-cedar heartwood, essential oil and wood constituents towards Phytophthora ramorum in culture. Forest Pathology, 36, 297–308;
29.
MatsuiN, OhiraT, HiramatsuY, ImaiY, MiyazakiY. (2007) The composition of volatiles from tatami mats containing hinoki (Chamaecyparis obtusa) wood-wool and its decline over the long term. Journal of Wood Science, 53, 529–532;
30.
ParkMJ, ChoiWS, KangHY, GwakKS, LeeGS, JeungEB, ChoiIG. (2010) Inhibitory effect of the essential oil from Chamaecyparis obtusa on the growth of food-borne pathogens. The Journal of Microbiology, 48, 496–501.
31.
ParkIK, LeeSG, ChoiDH, ParkJD, AhnYJ. (2003) Insecticidal activities of constituents identified in the essential oil from leaves of Chamaecyparis obtusa against Callosobruchus chinensis (L.) and Sitophilus oryzae (L.). Journal of Stored Products Research, 39, 375–384.
32.
SmithECJ, WilliamsonEM, WarehamN, KaatzGW, GibbonsS. (2007) Antibacterials and modulators of bacterial resistance from the imamture cones of Chamaecyparis lawsoniana. Phytochemistry, 68, 210–217;
33.
TuckerA, MaciarelloM, KarchesyJ. (2000) Commercial “rose of cedar” oil, the wood oil of Port Orford cedar, Chamaecyparis lawsoniana (A. Murray) Parl. (Cupressaceae). Journal of Essent Oil Research, 12, 24–26.
34.
XiaoD, KuroyanagiM, ItaniT, MatsuuraH, UdayamaM, MurakamiM, UmeharaK, KawaharaN. (2001) Studies on constituents from Chamaecyparis pisifera and antibacterial activity of diterpenes. Chemical & Pharmaceutical Bulletin, 49, 1479–1481.
35.
YangJK, ChoiMS, SeoWT, RinkerDL, HanSW, CheongGW. (2007) Chemical composition and antimicrobial activity of Chamaecyparis obtusa leaf essential oil. Fitoterapia, 78, 149–152;
36.
YatagaiM, NakataniN. (1994) Antimite, antifly, antioxidative and antibacterial activities of pisiferic acid and its congeners. Mokuzai Gakkaishi, 40, 1355–1362;
37.
Palá-PaúlJ, Pérez-AlonsoMJ, Velasco-NegueruelaA, Palá-PaúlR, SanzJ, ConejeroF. (2001) Seasonal variation in chemical constituents of Santolina rosmarinifolia L. ssp. rosmarinifolia. Biochemical Systematics and Ecology, 297, 663–672;
38.
Palá-PaúlJ, UsanoJ, SoriaAC, Pérez-AlonsoMJ, BrophyJJ. (2008) Essential oil composition of Eryngium campestre L. growing in different soil types. A preliminary study. Natural Product Communications, 3, 1121–1126.
39.
Peréz-AlonsoMJ, Velasco-NegueruelaA, Palá-PaúlJ, SanzJ. (2003) Variations in the essential oil composition of Artemisia pedemontana gathered in Spain: chemotype camphor-1,8-cineole and chemotype davanone. Biochemical Systematics and Ecology, 31, 77–84.
40.
Real Farmacopea Española, 3a Ed. (2005) Ministerio de Sanidad y Consumo, Madrid.
41.
Palá-PaúlJ, Usano-AlemanyJ, BrophyJJ, Pérez-AlonsoMJ, SoriaAC. (2010) Essential Oil Composition of the Different Parts of Eryngium aquifolium from Spain. Natural Product Communications, 5, 817–821.
42.
AdamsRP. (1995) Identification of essential oil components by gas chromatography/mass spectroscopy. Allured Publ., Carol Stream IL.
43.
Adams RP. (2001) Identification of essential oil components by gas chromatography/quadrupole mass spectroscopy. Allured Publ., Carol Stream IL;
44.
HellerSR, MilneGWA. (1978, 1980, 1983) US Enviromental Protection Agency (EPA)/US National Institutes of Health (NIH) Mass Spectral Data Base. US Government Printing Office, Washington, DC.;
45.
JoulainD, KönigAW. (1998) The atlas of spectral data of sesquiterpene hydrocarbons, E.B.-Verlag Hamburg;
46.
StenhagenE, AbrahamssonS, McLaffertyFW. (1974) Registry of mass spectral data, Wiley, New York;
