Ajowan is a popular spice that is used in folk medicine in Algeria. Most studies reported that thymol is the main component of the seed oil. Isothymol was reported to be a constituent of essential oil isolated from aerial parts. Using plants harvested in five different locations in north western Algeria, every two weeks during the flowering stage, we demonstrated that isothymol was the major component when the plant was collected near the sea at low altitude and at the beginning of the flowering stage. In other cases, thymol is the predominant constituent.
QuezelP, SantaS. (1963) Nouvelle flore de l'Algérie et des régions désertiques méridionales. Ed. CNRS, T. II, Paris, 671.
2.
ZachariahTJ. (2008) Ajowan. In Chemistry of Spices, ParthasarathyVA, ChempakamB, ZachariahTJ (Eds), CAB International, 312–318.
3.
SinghG, MauryaS, CatalanC, DelampasonaMP. (2004) Chemical constituents, antifungal and antioxidative effects of Ajwain essential oil and its acetone extract. Journal of Agricultural and Food Chemistry, 52, 3292–3296.
KoedamA, SchefferJJC, BaerheimSvendsen A. (1979) Comparison of isolation procedures for essential oils. Zeitschrift für Lebensmiitteluntersuchung und Forschung A, 168, 106–111.
9.
GersbachPV, ReddyN. (2002) Non-invasive localization of thymol accumulation in Carum copticum (Apiaceae) fruits by chemical shift selective magnetic resonance imaging. Annals of Botany, 90, 253–257.
Ill-MinC, TranDang K, Oh-KyuL, AteequeA. (2007) Chemical constituents from Ajawain seeds (Trachyspermum ammi) and inhibitory activity of thymol, lupeol and fatty acids on barnyardgrass and radish seeds. Asian Journal of Chemistry, 19, 1524–1534.
12.
KhajehM, YaminiY, SefidkonF, BahramifarN. (2004) Comparison of essential oil composition of Carum copticum obtained by supercritical carbon dioxide extraction and hydrodistillation methods. Food Chemistry, 86, 587–591.
13.
LucchesiME, ChematF, SmadjaJ. (2004) An original solvent free microwave extraction of essential oils from spices. Flavour Fragrance Journal, 19, 134–138.
14.
SrivastavaM, SaxenaA, BabyP. (1999) GC-MS investigation and antimicrobial activity of the essential oil of Carum copticum Benth. & Hook. Acta Alimentaria, 28, 291–295.
15.
ChoudhuryS, AhmedR, KanjilalPB, LeclercqPA. (1998) Composition of the seed oil of Trachyspermum ammi (L.) Sprague from Northeast India. Journal of Essential Oil Research, 10, 588–590.
16.
RasooliI, HadiFakoor M, YadegariniaD, GachkarL, AllamehA, Bagher RezaeiM. (2008) Antimycotoxigenic characteristics of Rosmarinus officinalis and Trachyspermum copticum L. essential oils. International Journal of Food Microbiology, 122, 135–139.
17.
LaouerH, ZerrougMM, SahliF, ChakerAN, ValentiniG, FerrettiG, GrandeM, AnayaJ. (2003) Composition and antimicrobial activity of Ammoïdes pusilla (Brot.) Breistr. essential oil. Journal of Essential Oil Research, 15, 135–138.
18.
KamboucheN, El-AbedD. (2003) Composition of the volatile oil from the aerial parts of Trachyspermum ammi (L.) Sprague from Oran (Algeria). Journal of Essential Oil Research, 15, 39–40.
19.
EzoubeiriA, GadhiCA, FdilN, BenharrefA, JanaM, VanhaelenM. (2005) Isolation and antimicrobial activity of two phenolic compounds from Pulicaria odora L. Journal of Ethnopharmacology, 99, 287–292.
20.
TomiF, BradesiP, BighelliA, CasanovaJ. (1995) Computer-aided identification of individual components of essential oils using carbon-13 NMR spectroscopy. Journal of Magnetic Resonance Analysis, 1, 25–34.
21.
RezziS, BighelliA, CastolaV, CasanovaJ. (2002) Direct identification and quantitative determination of acidic and neutral diterpenes using 13C NMR spectroscopy. Journal Applied Spectroscopy.56, 312–317.
22.
TomiF, CasanovaJ. (2006) 13C NMR as a tool for identification of individual components of essential oils from Labiatae. Acta Horticulturae, 723, 185–192.