Elucidation of the main structural unit of a water-soluble, high-molecular weight preparation from the crude polysaccharides of Anchusa italica Retz. roots has been carried out. According to 13C NMR, 1H NMR and 2D heteronuclear 1H/13C HSQC spectral data, the main structural element of the high-molecular, water-soluble preparation was a regularly substituted polyoxyethylene chain, namely poly[oxy-1-carboxy-2-(3,4-dihydroxyphenyl)ethylene]. Most carboxylic groups of this caffeic acid-derived polymer of A. italica are methylated.
BarbakadzeVV, KemertelidzeEP, TargamadzeIL, ShashkovAS, UsovAI. (2002) Poly[3-(3,4-dihydroxyphenyl)glyceric acid]: A new biologically active polymer from two comfrey species Symphytum asperum and S. caucasicum (Boraginaceae). Russian Journal of Bioorganic Chemistry, 28, 326–330.
2.
BarbakadzeV, van den BergAJJ, BeukelmanCJ, KemminkJ, Quarles vanUfford HC. (2009) Poly[3-(3,4-dihydroxyphenyl)-glyceric acid] from Symphytum officinale roots and its biological activity. Chemistry of Natural Compounds, 45, 6–10.
3.
BarthomeufCM, DebitonE, BarbakadzeVV, KemertelidzeEP. (2001) Evaluation of the dietetic and therapeutic potential of a high molecular weight hydroxicinnamate-derived polymer from Symphytum asperum Lepech. Regarding its antioxidant, antilipoperoxidant, antiinflammatory, and cytotoxic properties. Journal of Agricultural and Food Chemistry, 49, 3942–3946.
4.
BarbakadzeV, KemertelidzeE, TargamadzeI, MulkijanyanK, ShashkovAS, UsovAI. (2005) Poly[3-(3,4-dihydroxyphenyl)glyceric acid], a new biologically active polymer from Symphytum asperum Lepech. and S.caucasicum Bieb. (Boraginaceae). Molecules, 10, 1135–1144.
5.
BarbakadzeVV, KemertelidzeEP, MulkijanyanKG, van den BergAJJ, BeukelmanCJ, van den WormE, Quarles vanUfford HC, UsovAI. (2007) Antioxidant and anticomplement activity of poly[3-(3,4-dihydroxyphenyl)glyceric acid] from Symphytum asperum and S. caucasicum plants. Pharmaceutical Chemistry Journal, 41, 14–16.
6.
KardavaL, GabuniyaKh, TevzadzeM, BarbakadzeV, GhirdaladzeD, IosavaG, PorakishviliN. (2000) Dihydroxycinnamate-derived polymer from Symphytum asperum increases spontaneous in-vitro apoptosis of B-chronic lymphocytic leukaemia cells. Bulletin of the Georgian Academy of Sciences, 162, 47–50.
7.
KardavaL, KulikovaN, TevzadzeM, GabuniyaKh, BarbakadzeV, GhirdaladzeD, IosavaG, PorakishviliN. (2001) The cell cycle progression of B-chronic lymphocytic leukaemia cells in-vitro. Proceedings of the Georgian Academy of Sciences, 27, 465–470.
8.
BarbakadzeV, MulkijanyanK, MerlaniM, GogilashviliL, AmiranashviliL, Vidal-VanaclochaF. (2008) Effects of poly[3-(3,4-dihydroxyphenyl)glyceric acid] on the inflammatory response of tumor-activated hepatic sinusoidal endothelium. Bulletin of the Georgian National Academy of Sciences, 2, 108–112.
9.
BarbakadzeVV, GakhokidzeRA, ShengeliaZS, UsovAI. (1989) Preliminary investigation of water-soluble polysaccharides of some plants of Georgia. Chemistry of Natural Compounds, 25, 281–286.
10.
BarbakadzeVV, KemertelidzeEP, DekanosidzeGE, BeruchashviliTG, UsovAI. (1992) Investigation of glucofructans from roots of two species of comfrey Symphytum asperum Lepech. and S. caucasicum Bieb. Bioorganicheskaya Khimiya, 18, 671–679 (in Russian).
11.
OdonmazigP, BadgaD, EbringerovaA, AlfoldiJ. (1992) Structures of pectic polysaccharides isolated from the Siberian apricot (Armeniaca siberica Lam.). Carbohydrate Research, 226, 353–358.
12.
DuanJ, ZhengY, DongQ, FangJ. (2004) Structural analysis of a pectic polysaccharide from the leaves of Diospyros kaki. Phytochemistry, 65, 609–615.
13.
KesterHCM, MagaudD, AnkerCRD, DoutheauA, ShevchikV, Hugouvieux-Cotte-PattatN, BenenJAE, VisserJ. (1999) Performance of selected microbial pectinases on synthetic monomethyl-esterified di- and trigalacturonates. The Journal of Biological Chemistry, 274, 37053–37059.
14.
DupauP, EppleR, ThomasAA, FokinVV, SharplessKB. (2002) Osmium-catalyzed dihydroxylation of olefins in acidic media: old process, new tricks. Advanced Synthesis and Catalysis, 344, 421–433.
15.
PardiniVL, SakataSK, VargasRR, ViertlerH. (2001) Anodic methoxylation of cinnamate esters. Journal of the Brazilian Chemical Society, 12, 223–229.
16.
VignonMR, Garcia-JaldonC. (1996) Structural features of the pectic polysaccharides isolated from retted hemp bast fibres. Carbohydrate Research, 296, 249–260.
17.
PolleAYa, OvodovaRG, ShashkovAS, OvodovYS. (2002) Some structural features of pectic polysaccharide from tansy, Tanacetum vulgare L. Carbohydrate Polymers, 49, 337–344.
18.
HabibiY, MahrouzM, VignonMR. (2005) Isolation and structural characterization of protopectin from the skin of Opuntia ficus indica prickly pear fruits. Carbohydrate Polymers, 60, 205–213.
19.
WuD, ChenA, JohnsonCSJr. (1995) An improved diffusion-ordered spectroscopy experiment incorporating bipolar-gradient pulses. Journal of Magnetic Resonance A, 115, 260–264.
20.
PiottoM, BourdonneauM, ElbayedK, WieruszeskiJ-M, LippensG. (2006) New DEFT sequences for the acquisition of one-dimensional carbon NMR spectra of small unlabelled molecules. Magnetic Resonance in Chemistry, 44, 943–947.
21.
BarbakadzeV, KemertelidzeE, UsovAI, KroesBH, Quarles van UffordHC, van den WormE, BeukelmanCJ, van den BergAAJ, LabadieRP. (1999) Evaluation of immunomodulatory activity of some plant polysaccharides. Proceedings of Georgian Academy of Sciences, Biological Series, 25, 207–216.
22.
DuboisM, GillesKA, HamiltonJK, RebersPA, SmithF. (1956) Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 28, 350–356.
23.
YapheW, ArsenaultGP. (1965) Improved resorcinol reagent for the determination of fructose, and of 3,6-anhydrogalactose in polysaccharides. Analytical Biochemistry, 13, 143–148.
24.
BlumenkrantzN, Asboe-HansenG. (1973) New method for quantitative determination of uronic acid. Analytical Biochemistry, 54, 484–489.
25.
ShcherbukhinVD, MironovaLI, KondyrevaAV, GryunerVS. (1970) Determination of glucose in molasses by the glucose oxidase method using potassium ferrocyanide. Applied Biochemistry and Microbiology, 6, 467–470 (in Russian).
26.
YorkWS, DarvilAG, McNeilMA, StevensonTT, AlbersheimP. (1985) Isolation and characterization of plant cell walls and cell-wall components. Methods in Enzymology, 118, 3–40.