Gmelinoside I (1), a new flavonol glycoside, was isolated and chemically characterized as 3,5,7,3′,4′,5′-hexahydroxy-3-O-α-D-galactopyranosylflavone from the aerial part of Limonium gmelinii Kuntze (Plumbaginaceae).. Other compounds were identified as (-)-epigallocatechin-3-O-gallate, myricetin, myricetin-3-O-α-L-rhamnopyranoside, myricetin-3-O-β-D-galactoside-6″-O-gallate and caprolactam. The antibacterial, antifungal and antileishmanial activities of the isolated compounds were evaluated. Myricetin showed moderate antileishmanial activity.