A two-step process has been developed for the purification of nicotine from waste tobacco using an aqueous two-phase system and solvent reverse extraction. The partitioning behavior of nicotine was investigated in an isopropyl alcohol/ (NH4)2SO4 aqueous two-phase system. The effects of the addition of salt, pH and temperature on the partitioning behavior of nicotine were studied. The recovery rate of nicotine was 96.1% with a purity of above 99% when optimal conditions were used [(NH4)2SO4 25%, pH 9, temperature 35°C, isopropyl alcohol 5 mL].
BhatCV, SindhiPC. (1993) Ion exchange column studies with nicotine choice of solvent and flow rate, Oriental Journal of Chemistry, 9, 246–254;
2.
DimitrisP, NikolaosEL. (2006) Development of an aqueous two-phase partitioning system for fractionating therapeutic proteins from tobacco extract, Journal of Chromatography A, 1128, 114–124;
3.
MillenDLC, MurphyWR. (1993) Countercurrent extraction of nicotine from tobacco juice, Industrial & Engineering Chemistry Research, 32, 3056–3060;
4.
HanF, TangG. (1995) Study on preparation of nicotine from waste tobacco, Modern Chemical Industry, 6, 30–32;
5.
NarasimhaRCV, ChakrabortyMK. (1992) Preliminary studies with ion exchange resins for recovery nicotine from tobacco waste, Research and Industry, 37, 115–123;
6.
LiSL, YuZL, LiGF., (2004) New technology for extraction nicotine, Fine Chemistry, 21, 439–441.
7.
ZhangC, FabricioMB, ScottB, CramerCL. (2005) Purification and stabilization of ricin B from tobacco hairy root culture medium by aqueous two-phase extraction, Journal of Biotechnology, 117, 39–48;
8.
MianL, PeeplesTL. (2004) Purification of hyperthermophilic archaeal amylolytic enzyme (MJA1) using thermoseparating aqueous two-phase systems, Journal of Chromatography B, 807, 69–74;
9.
FarshadR, GashawM, FarzanehF., (2007) Optimizing refolding and recovery of acing surface response analysis, Journal of Chromatography A, 1141, 32–40;
10.
BoraMM, BorthakurS, RaoPC., (2005) Aqueous two-phase partitioning of cephalosporin antibiotics: effect of solute chemical nature, Separation and Purification Technology, 45, 153–156;
11.
ZhaiSL, LuoGS, LiuJG., (2001) Aqueous two-phase electrophoresis for separation of amino acids, Separation and Purification Technology, 21, 197–203.