Phytochemical investigations of multiple shoot cultures of selected accessions AGB002 and AGB025 of Withania somnifera. established in vitro utilizing shoot tip apices cultured on Murashige and Skoog's medium supplemented with BAP (1 mg/L) have been carried out. This has lead to isolation of four glycowithanolides viz. Withanoside IV (WSG-3), Withanoside VI (WSG-3A), Physagulin D (WSG-P) and Withastraronolide (WSC-O). The structures of these have been confirmed on the basis of spectroscopic data. Multiple shoot cultures could be an alternative renewable resource for production of these biologically active molecules.
AtalCK, GuptaOP, RagunathanK, DharKL. (1975) Pharmacognosy and phytochemistry of Withania somnifera (Linn.)Dunal. Central Council of Research in Indian Medicine and Homeopathy.New Delhi, 6–18.
2.
AgarwalR., DiwanagS, PatkiP, PatwardhanB.(1999) Studies on immunomodulartory activity of Withania somnifera (Ashwagandha) extracts in experimental immune inflammation. Journal of Ethnopharmacology, 67, 27–35.
3.
MishraLC, SinghBB, DagenaisS. (2000) Scientific basis for therapeutic uses of Withania somnifera (Ashwagandha): A review. Alternative Medicine Review, 5, 334–345.
4.
BudhirajaRD, SudhirS. (2000) Review of biological activity of withanolides (antibacterial, antitumor, immunomodulating, anti-inflammatory and insect antecedent)Journal of Scientific and Industrial Research, 46, 488–491.
5.
SinghS, KumarS. (1998) Withania somnifera – The Indian ginseng- ashwagandha.Central Institute of Medicinal and Aromatic Plants, Lucknow, India.
6.
RamchandraRao S, RavishankarGA. (2002) Plant cell cultures: chemical factories of secondary metabolites. Biotechnology Advances, 20, 101–153.
7.
VanisreeMulabagal, Hsin-ShengTsay. (2004) Plant Cell Cultures - An alternative and efficient source for production of biologically important secondary metabolites. International Journal of Applied. Sciences and Engineering, 2, 29–48.
8.
RojaG, HebleMR, SipahimaliniAT. (1991) Tissue cultures of Withania somnifera: Morphogenesis and withanolide synthesis. Physiotherapy Research, 5, 185–187.
9.
SharadaM, AhujaA, SuriKA, VijSP, KhajuriaRK, VermaV, KumarA. (2007) Withanolide production by in vitro cultures of Withania somnifera (L) Dunal and its association with differentiation. Biologia Plantarum, 51, 161–164.
10.
SharadaM, AhujaA, VijSP (2008) Application of biotechnology in Indian ginseng (ashwagandha): progress and prospects. In Recent Advances in Plant BiotechnologyAshwaniKumar, SoporySK. (Eds.) IK International Pvt. Ltd., New Delhi, India, 561–585.
11.
BhattacharyaSK, SatyanKS, GhosalS. (1997) Antioxidant activity of glycowithanolides from Withania somnifera. Indian Journal Experimental Biology, 35, 463–469.
12.
BhattachayaSK, BhattacharyaA, SairamK, GhosalS. (2000) Anxiolytic-antidepressant activity of Withania somnifera Glycowithanolides: an experimental study. Phytomedicine, 74, 63–69.
13.
BhattacharyaSK, KumarA, GhosalS. (1995) Effects of glycowithanolides from Withania somnifera on an animal model of alzheimer's disease and perturbed central cholinergic markers of cognition in rats. Phytotherapy Research, 9, 110–113.
14.
GhosalS, LalJ, SrivastavaRS. (1988) Immunomodulatory and CNS effect of sitoindosides IX and X, two regio glycowithanolides from Withania somnifera. Indian Journal of Natural Products, 4, 12–13.
15.
RayAB, GuptaM. (1994) Withasteroids, a growing group of naturally occurring steroidal lactones. –In Progress in the Chemistry of Organic Natural Products.HerzW, KirbyGW, MooreRE, SteglichW, TammC. (Eds.) Springer-Verlag, New York, 1–106.
16.
RayS, JhaS. (2001) Production of withaferin A in shoot cultures of Withania somnifera, Planta medica, 67, 432–436.
17.
MurashigeT, SkoogF. (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiologia Plantarum, 15, 473–497.
