The stability of salvianolic acid B and total phenolic acids from Salvia miltiorrhiza in water solutions at different temperatures, in buffered aqueous solutions at different pHs and in biological fluids, including simulated gastric and intestinal fluids, were investigated in vitro. The results showed that the degradation of salvianolic acid B was pH- and temperature-dependent. Furthermore, structures of the degradation products of salvianolic acid B and total phenolic acids were elucidated by liquid chromatography-electrospray ion trap mass spectrometry and analysis of the degraded solutions revealed seventeen degradation products. The possible degradation pathway of salvianolic acid B is proposed.
JiXY, TanBK, ZhuYC, LinzW, ZhuYZ. (2003) Comparison of cardioprotective effects using ramipril and Danshen for the treatment of acute myocardial infarction in rats. Life Sciences, 73, 1413–1426.
2.
LiuJ, ShenHM, OngCN. (2000) Salvia miltiorrhiza inhibits cell growth and induces apoptosis in human hepatoma HepG2 cells. Cancer Letters, 153, 85–93.
3.
WuXJ, WangYP, WangW, SunWK, XuYM, XuanLJ. (2000) Free radical scavenging and inhibition of lipid peroxidation by magnesium lithospermate B. Acta Pharmacologica Sinica, 21, 855–858.
4.
ZhaoSJ, ZhangGY, LiuD, HuZB. (2004) Advances in research of pharmacological activities and biosynthesis pathway of water-soluble phenolic compounds of Salvia miltiorrhiza. Chinese Traditional and Herbal Drugs, 35, 341–344.
5.
LingHY, LuXZ, ZhaoYL, ZhangSW. (1999) The survey on the research of water-soluble components of Chinese medicine “Danshen”. Natural Product Research and Development, 1, 75–81.
6.
LiuAH, LiL, XuM, LinYH, GuoHZ, GuoDA. (2006) Simultaneous quantification of six major phenolic acids in the roots of Salvia miltiorrhiza and four related traditional Chinese medicinal preparations by HPLC-DAD method. Journal of Pharmaceutical and Biomedical Analysis, 41, 48–56.
7.
ZhouCX, LuoHW, NiwaM. (1999) Isolation and identification of water-soluble constituents of Salvia miltiorrhiza. Journal of China Pharmaceutical University, 30, 411–416.
8.
ZhangY, AkaoT, NakamuraN, HattoriM, YangXW, DuanCL, LiuJX. (2004) Magnesium lithospermate B is excreted rapidly into rat bile mostly as methylated metabolites, which are potent antioxidants. Drug Metabolism and Disposition, 32, 752–757.
9.
ChenYL, HuCS, LinFY, ChenYH, SheuLM, KuHH, ShiaoMS, ChenJW, LinSJ. (2006) Salvianolic acid B attenuates cyclooxygenase-2 expression in vitro in LPS-treated human aortic smooth muscle cells and in vivo in the apolipoprotein-E-deficient mouse aorta. Journal of Cellular Biochemistry, 98, 618–631.
10.
LinYH, LiuAH, WuHL, WestenbroekC, SongQL, YuHM, Ter HorstGJ, LiXJ. (2006) Salvianolic acid B, an antioxidant from Salvia miltiorrhiza, prevents Aβ25-35-induced reduction in BPRP in PC12 cells. Biochemical and Biophysical Research Communications, 348, 593–599.
11.
YokozawaT, LeeTW, ChungHY, OuraH, NonakaG, NishiokaI. (1994) Depressor effect of magnesium lithospermate B, a component of Salviae Miltiorrhizae Radix, in spontaneously hypertensive rats. Phytotherapy Research, 8, 271–275.
12.
YueXD, QuGW, LiGS, ZangYH, ChiZM. (2005) Stability of salvianolic acid B in water solution. Chinese Traditional and Herbal Drugs, 36, 205–207.
13.
ChaiSW, PanGX, ZhangYB. (2006) Stability investigation of salvianolic acid B in simulated body fluid of digestive tract. Tianjin Journal of Traditional Chinese Medicine, 23, 332–333.
14.
YeM, YanYN, GuoDA. (2005) Characterization of phenolic compounds in the Chinese herbal drug Tu-Si-Zi by liquid chromatography coupled to electrospray ionization mass spectrometry. Rapid Communications in Mass Spectrometry, 19, 1469–1484.
15.
LiuRX, YeM, GuoHZ, BiKS, GuoDA. (2005) Liquid chromatography/electrospray ionization mass spectrometry for the characterization of twenty-three flavonoids in the extract of Dalbergia odorifera. Rapid Communications in Mass Spectrometry, 19, 1557–1565.
16.
YangM, LiuAH, GuanSH, SunJH, XuM, GuoDA. (2006) Characterization of tanshinones in the roots of Salvia miltiorrhiza (Dan-shen) by high-performance liquid chromatography with electrospray ionization tandem mass spectrometry. Rapid Communications in Mass Spectrometry, 20, 1266–1280.
17.
ZhangJL, CuiM, HeY, YuHL, GuoDA. (2005) Chemical fingerprint and metabolic fingerprint analysis of Danshen injection by HPLC-UV and HPLC-MS methods, Journal of Pharmaceutical and Biomedical Analysis, 36, 1029–1035.
18.
YeM, GuoDA, YeG, HuangCG. (2005) Analysis of homoisoflavonoids in Ophiopogon japonicus by HPLC-DAD-ESI-MSn, Journal of the American Society for Mass Spectrometry, 16, 234–243.
19.
YeM, HanJ, ChenHB, ZhengJH, GuoDA. (2007) Analysis of phenolic compounds in Rhubarbs using liquid chromatography coupled with electrospray ionization mass spectrometry. Journal of the American Society for Mass Spectrometry, 18, 82–91.
20.
LiuAH, LinYH, YangM, GuoH, GuanSH, SunJH, GuoDA. (2007) Development of the fingerprints for the quality of the roots of Salvia miltiorrhiza and its related preparations by HPLC-DAD and LC-MSn. Journal of Chromatography B, 846, 32–41.
21.
HanJ, YeM, XuM, SunJH, WangBR, GuoDA. (2007) Characterization of flavonoids in the traditional Chinese herbal medicine HuangQin by liquid chromatography coupled with electrospray ionization mass spectrometry, Journal of Chromatography B, 848, 355–362.
22.
GuoH, LiuAH, YeM, YangM, GuoDA. (2007) Characterization of phenolic compounds in the fruits of Forsythia suspensa by high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry, Rapid Communications in Mass Spectrometry, 21, 715–729.
23.
YangM, WangXM, GuanSH, XiaJM, SunJH, GuoH, GuoDA. (2007) Analysis of triterpenoids in Ganoderma lucidum using liquid chromatography coupled with electrospray ionization mass spectrometry. Journal of the American Society for Mass Spectrometry, 18, 927–939.
24.
SunJH, YangM, WangXM, XuM, LiuAH, GuoDA. (2007) Identification of tanshinones and their metabolites in rat bile after oral administartion of TTE-50, a standardized extract of Salvia miltiorrhiza by HPLC-ESI-DAD-MSn. Journal of Pharmaceutical and Biomedical Analysis, 44, 564–574.
25.
HanJ, YeM, HuoH, YangM, WangBR, GuoDA. (2007) Analysis of multiple constituents in a Chinese herbal preparation Shuang-Huang-Lian oral liquid by HPLC-DAD-ESI-MSn. Journal of Pharmaceutical and Biomedical Analysis, 44, 430–438.
26.
LiuAH, GuoH, YeM, LinYH, SunJH, XuM, GuoDA. (2007) Detection, characterization and identification of phenolic acids in Danshen using high-performance liquid chromatography with diode array detection and electrospray ionization mass spectrometry. Journal of Chromatography A, 1161, 170–182.
27.
National Commission of Chinese Pharmacopoeia. (2005) Pharmacopoeia of People's Republic of China. Chemical Industry Press, Beijing, 2, appendix 72.
