Prosopis glandulosa Torr. var. torreyana (L. Benson) M. C. Johnston root bark, collected in Texas, yielded two new alkylated piperidine alkaloids, namely 3-isocassine (1) and N-methyl-3-isocassine (2), together with the previously reported N-methylcassine (3). In addition, the alkylated piperidinyl indolizidine alkaloid juliprosopine (4) was isolated from the leaves of the same plant. The structures and stereochemistry of compounds 1–4 were determined from 1D and 2D NMR data, including COSY, HMQC, HMBC and NOESY experiments, and ESI-HRMS. Juliprosopine (4) demonstrated antimicrobial activities against a panel of microorganisms, including potent antifungal activities against Aspergillus fumigatus.
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