Pumpkin seeds are used in the treatment of benign prostatic hyperplasia and the active constituents have been reported to be the sterols and fatty acids. In this study, the oils extracted from the two seed samples of pumpkin (Cucurbita pepo L. ssp. pepo) growing in Turkey were analyzed for their fatty acid contents. Fatty acids were determined by capillary gas chromatographymass spectrometry (GC-MS) equipped with a flame ionization detector. The results showed that both samples, which had very similar fatty acid compositions, were quite rich in linoleic acid (43.3 and 43.8%), followed by oleic (36.8 and 35.8%), palmitic (11.9 and 10.9), and stearic acids (6.7 and 7.6%). Saponification, esterification, iodine, peroxide, acidification indexes, and unsaponifiable matter of the oils were also calculated. In addition, the two oil samples were tested for their antibacterial, antifungal, and antiviral activities. Both of the oils displayed the same activity profile, having a good antibacterial activity against Klebsiella pneumoniae and Acinobacter baumannii (16 μg/mL), potent antifungal activity against Candida albicans (8 μg/mL), as well as a moderate antiviral effect towards Parainfluenza virus type-3 (16–8 μg/mL).
BuckAC. (1996) Phytotherapy for prostate. British Journal of Urology, 78, 325–336.
2.
DreikornK. (2002) The role of phytotherapy in treating lower urinary tract symptoms and benign prostatic hyperplasia. World Journal of Urology, 19, 426–435.
3.
MeyerJP, GillattDA. (2002) Alternative medications for benign prostatic hyperplasia available on the internet: a review of the evidence for their use. BJU International, 90, 41–44.
4.
ChappleCR. (2004) Pharmacological therapy of benign prostatic hyperplasia/lower urinary tract symptoms: an overview for the practising clinician. BJU International, 94, 738–744.
5.
Al ZuhairH, El FattahAA, El SayedMI. (2000) Pumpkin seed oil modulates the effect of felodipine and captopril in spontaneously hypertensive rats. Pharmacological Research, 41, 555–563.
6.
PDR (Physician's Desk Reference) for Herbal Medicines (2004) 3rd Edition. Thomson, pp. 663–665.
7.
MurkovicM, HillebrandA, WinklerJ, LeitnerE, PfannhauserW. (1996) Variability of fatty acid content in pumpkin seeds (Cucurbita pepo L.). European Food Research and Technology, 203, 216–219.
8.
YounisYMH, GhirmayS, Al-SishrySS. (2000) African Cucurbita pepo L. properties of seed and variability in fatty acid composition of seed oil. Phytochemistry, 54, 71–75.
9.
MurkovicM, PfannhauserW. (2000) Stability of pumpkin seed oil. European Journal of Lipid Science Technology, 102, 607–611.
10.
El-AdawyTA, TahaKM. (2001) Characteristics and composition of different seed oils and flours. Food Chemistry, 74, 47–54.
11.
ApplequistWL, AvulaB, SchanebergBT, WangYH, KhanIA. (2006) Comparative fatty acid content of seeds of four Cucurbita species grown in a common (shared) garden. Journal of Food Composition and Analysis, 19, 606–611.
12.
MorrisonWR, SmithLM. (1964) Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron trifluoride-methanol. Journal of Lipid Research, 5, 600–608.
13.
OzcelikB, OrhanI, TokerG (2006) Antiviral and antimicrobial assesment of some flavonoid type of compounds. Zeitschrift Naturforschung Section C, 61c, 632–638.
14.
ŞenerB, OrhanI, OzcelikB. (2007) Diterpenoid Alkaloids from some Turkish Consolida species and their antiviral activities. ARKIVOC, 7, 265–272.
15.
OrhanI, OzcelikB, KaraogluT, AslanS, KartalM, ŞenerB. (2006) Antibacterial, Antifungal, Antiviral and Antioxidant Potential of Lycopodium clavatum L. Phytochemistry Reviews (in press).
16.
ÖzçelikB, AslanM, OrhanI, KaraogluT. (2005) Antibacterial, antifungal, and antiviral activities of the lipophylic extracts of Pistacia vera L. Microbiological Research, 160, 159–164.
17.
FreyHR, LiessB. (1971) Vermehrungskinetik und Werwendbarkeit eines stark zytopathogenen VD-MD virusstammes für diagnostische untersuchungen mit der mikrotiter-methode. Journal of Veterinary Medicine Series B, 18, 61–71.
18.
ÖzçelikB, KüsmenogluŞ, TürközS, AbbasogluU. (2004) Antimicrobial activities of some plants from the Apiaceae family. Pharmaceutical Biology, 42, 526–528.
