Ciprofloxacin, Erythromycin and Norfloxacin are potent anti-microbials. These antibiotics, besides affecting bacteria, also exert toxic effects on mammalian cells. The present work was undertaken to study the effect of these antibiotics on the osmotic fragility profile of erythrocytes and the protective role played by vitamin supplementation, which exerts a stabilizing effect on the erythrocytes making them more resistant to lysis under hypotonic stress. The protective effect of β-carotene and L-ascorbic acid was significant (p < 0.01) at 0.1 mM and 0.01 mM concentrations. No significant effect was observed at lower concentrations. The free radical scavenging activity and effect of these vitamins on membrane fluidity and permeability may explain the protective role against the damage induced by the antibiotics on the erythrocytes membrane.
BeutlerE. (1971) Abnormalities of the hexose monophosphate shunt. Seminar in Hematology, 8, 311–347.
2.
U S Department of the Army TM 8-227-4. (1963) Laboratory Procedures in Clinical Hematology, US Govt.Printing OfficeWashington, DC, 427–430.
3.
BurkeBE, ShottonDM. (1983) Erythrocyte membrane skeleton abnormalities in hereditary spherocytosis. British Journal of Hematology, 54, 173–187.
4.
SenG, MukopadhayaR, GhosalJ, BiswasT. (2004) Combination of ascorbate and α tocopherol as a preventive therapy against structural and functional defects of erythrocytes in visceral leishmaniasis. Free Radical Research, 38, 527–534.
5.
SirichotiyakulS, TantipalakornC, SanguansermsriT, WanapirakC, TongsongT (2004). Erythrocyte osmotic fragility test for screening of alpha-thalassemia-1 and beta-thalassemia trait in pregnancy. International Journal of Gynecology and Obstetrics, 86, 347–350.
6.
DesanctisR, De BelliSR, ScesaC, ManciniU, CucchiariniL, DuchaM. (2004) In vitro protective effect of Rhodiola rosea extract against hypochlorous acid-induced oxidative damage in human erythrocytes. Biofactor, 20, 147–159.
7.
RanjanR, SwarupD, NareshR, PatraRC. (2005) Enhanced erythrocyte lipid peroxides and reduced plasma ascorbic acid and alteration in blood trace elements level in dairy cows with mastitis. Veterinary Research Communication, 29, 27–34.
8.
DrabkoK, KuwalczykJ. (2004) Imbalance between pro-oxidative and anti-oxidative processes in children with neoplastic disease. Medycyna wieku rozwojowego, 8, 217–223.
9.
ShivarajashankaraYM, SivashankaraAR, BhatPG, RaoSH. (2003) Lipid peroxidation and antioxidant systems in the blood of young rats subjected to chronic fluoride toxicity. Indian Journal of Experimental Biology, 41, 857–860.
10.
NazriogluM, SimsekM, KutluM. (2004) Moderate exercise with a dietary vitamin C & E combination protects against streptozotocin-induced oxidative damage to the blood and improves faetal outcome in pregnant rats. Clinical Chemistry and Laboratory Medicine, 42, 511–517.
11.
VijayamaliniM, ManoharanS. (2004) Lipid peroxidation, vitamin C, E and reduced glutathione levels in patients with pulmonary tuberculosis. Cell Biochemistry and Function, 22, 19–22.
MalarkodiKP, SivaPrasad R, VaralakshmiP. (2004) Effect of lipoic acid on the oxidoreductive status of the red blood cells in rats subject to oxidative stress by chronic administration of Adriamycin. Human and Experimental Toxicology, 23, 129–135.
14.
AhmadI, SuhailM. (2002) Protective role of vitamin E on mefenamic acid-induced alterations in erythrocytes. Biochemistry, 67, 945–948.
15.
NagasawaT, KojimaS, KimuraE. (1982) Coil planet centrifugal and capillary tube centrifugal analysis of factors regulating erythrocyte osmotic fragility and deformability. Japanese Journal of Physiology, 32, 25–33.
16.
OrbanZ, MacDonaldLL, PetersMA, GuslitsB (1995) Erythromycin-induced cardiac toxicity. The American Journal of Cardiology, 75, 859–861.
17.
EtlikO, TomurA, DundarK, ErdemA, GundoganNU. (1997) The effect of anti-oxidant vitamins E and C on lipoperoxidation of erythrocyte membranes during hyperbaric oxygenation. Journal of Basic and Clinical Physiology and Pharmacology, 8, 269–277.
18.
EtlikO, TomurA, TuncerM, RidvanagaogluAY, AndacO. (1997) Protective effect of anti-oxidant vitamins on red blood cell lipoperoxidation induced by SO2 inhalation. Journal of Basic and Clinical Physiology and Pharmacology, 8, 31–43.
19.
GabbianelliR, NasutiC, FalcioniG, CantalmessaF. (2004) Lymphocyte DNA damage in rats exposed to pyrethroids: effect of supplementation with vitamin E and C. Toxicology, 203, 17–26.
20.
RifkindJM, ArakiK, HadleyEC. (1983) The relationship between the osmotic fragility of human erythrocytes and cell age. Archives of Biochemistry and Biophysics, 222, 582–589.
21.
ArakiK, RifkindJM. (1980) Age-dependent changes in osmotic hemolysis of human erythrocytes. Journal of Gerontology, 35, 499–505.
22.
ChanTK, LacellaPL, WeedRI. (1975) Slow phase hemolysis in hypotonic electrolyte solution. Journal of Cellular Physiology, 85, 47–57.
23.
BengaG, TravisBD, PopVI, PopescuO, TodderS, HolmesRP. (1984) The effect of saturation and isomerisation of dietary fatty acids on the osmotic fragility and water diffusional permeability of rat erythrocytes. Biochimica et Biophysica Acta, 775, 255–259.
24.
ArakiK, RifkindJM. (1981) The rate of osmotic hemolysis: A relationship with membrane bilayer fluidity. Biochimica et Biophysica Acta, 645, 81–90.
25.
Knopik-skrockaA, BielawskiJ, GlabM, KlafaczynskaA, WulkiewiczM. (2003) A kinetic study of pig erythrocyte hemolysis induced by polyene antibiotics. Cellular and Molecular Biology Letters, 8, 439–454.
26.
Knopik-skrockaA, BielawskiJ. (2002) The mechanism of the hemolytic activity of polyene antibiotics. Cellular and Molecular Biology Letters, 7, 31–48.
27.
BrajtburgJ, ElbergS, SchwartzDR, Vertut-CroquinA, SchlessingerD, KobayashiGS, MedoffG. (1985) Involvement of oxidative damage in erythrocyte lysis induced by Amphotericin B. Antimicrobial agents and Chemotherapy, 27, 172–176.
28.
De JongK, GeldwerthD, KuypersFA. (1997) Oxidative damage does not alter membrane phospholipids asymmetry in human erythrocytes. Biochemistry, 36, 6768–6776.
29.
LeeSK, LeeBM. (1997) Oxidation of erythrocyte protein and lipid and hemolysis in rabbit red blood cells treated with benzo[a] pyrene or Adriamycin. Journal of Toxicology and Environmental Health, 51, 557–569.
30.
ColinoCI, Garcia TurinoA, SanchezNavarro A, LanaoJM. (1998) A comparative study of Ofloxacin and ciprofloxacin in erythrocyte distribution. Biopharmaceutics and Drug Disposition, 19, 71–77.
