Considering the scarcity of literature data about the predictive capacity of polarization microscopy (PM) in identifying meiotic normality in human in vivo-matured oocytes, the main objective of the present study was to determine whether qualitative analysis of the spindle through PM can predict meiotic normality in fresh in vivo-matured human oocytes.
Methods:
Infertile patients undergoing controlled ovarian stimulation to intracytoplasmic sperm injection (ICSI) were selected. Fresh in vivo-matured oocytes were evaluated by PM and, immediately afterward, fixed for confocal microscopy (CM) analysis for evaluation of the spindle and chromosome distribution. We evaluated the percentage of oocytes with meiotic normality and abnormality determined by CM among oocytes with visible and nonvisible spindle and between oocytes with the spindle located at 0° to 60° angle to the first polar body (PB; normal position) and at 60° to 90° angle to the first PB (risky position) according to PM.
Results:
From 23 patients, 73 oocytes were analyzed. There were no significant differences in the percentage of oocytes with meiotic abnormalities among oocytes with visible and nonvisible spindles and among oocytes with the spindle in the normal and risky positions.
Conclusions:
Under the conditions tested, qualitative evaluation of the spindle through PM is not consistent with CM analysis and has limited predictive value of meiotic normality in fresh in vivo-matured human oocytes, which needs to be confirmed in larger studies. Our findings make questionable the usefulness of this methodology as a tool for noninvasive oocyte selection for ICSI.
MattsonBAAlbertiniDF. Oogenesis: chromatin and microtubule dynamics during meiotic prophase. Mol Reprod Dev. 1990;25(4):374–383.
2.
AlbertiniDF. Cytoplasmic microtubular dynamics and chromatin organization during mammalian oogenesis and oocyte maturation. Mutat Res. 1992;296(1-2):57–68.
3.
LiuLOldenbourgRTrimarchiJRKeefeDL. A reliable, noninvasive technique for spindle imaging and enucleation of mammalian oocytes. Nat Biotechnol. 2000;18(2):223–225.
4.
WangWHKeefeDL. Prediction of chromosome misalignment among in vitro matured human oocytes by spindle imaging with the PolScope. Fertil Steril. 2002;78(5):1077–1081.
5.
NavarroPALiuLTrimarchiJRFerrianiRAKeefeDL. Noninvasive imaging of spindle dynamics during mammalian oocyte activation. Fertil Steril. 2005;83suppl 1:1197–1205.
6.
MandelbaumJAnastasiouOLevyRGuerinJFde LarouziereVAntoineJM. Effects of cryopreservation on the meiotic spindle of human oocytes. Eur J Obstet Gynecol Reprod Biol. 2004;113 suppl 1:S17–S23.
7.
CoticchioGSciajnoRHuttKBromfieldJBoriniAAlbertiniDF. Comparative analysis of the metaphase II spindle of human oocytes through polarized light and high-performance confocal microscopy. Fertil Steril. 2010;93(6):2056–2064.
8.
HuYBetzendahlICortvrindtRSmitzJEichenlaub RitterU. Effects of low O2 and ageing on spindles and chromosomes in mouse oocytes from pre-antral follicle culture. Hum Reprod. 2001;16(4):737–748.
9.
Eichenlaub RitterUShenYTinnebergHR. Manipulation of the oocyte: possible damage to the spindle apparatus. Reprod Biomed Online. 2002;5(2):117–124.
10.
MullenSFAgcaYBroermannDCJenkinsCLJohnsonCACritserJK. The effect of osmotic stress on the metaphase II spindle of human oocytes, and the relevance to cryopreservation. Hum Reprod. 2004;19(5):1148–1154.
11.
RobertsRFranksSHardyK. Culture environment modulates maturation and metabolism of human oocytes. Hum Reprod. 2002;17(11):2950–2956.
12.
BattagliaDEGoodwinPKleinNASoulesMR. Influence of maternal age on meiotic spindle assembly in oocytes from naturally cycling women. Hum Reprod. 1996;11(10):2217–2222.
13.
Van BlerkomJHenryG. Oocyte dysmorphism and aneuploidy in meiotically mature human oocytes after ovarian stimulation. Hum Reprod. 1992;7(3):379–390.
14.
JonesKTLaneSI. Chromosomal, metabolic, environmental, and hormonal origins of aneuploidy in mammalian oocytes. Exp Cell Res. 2012;318(12):1394–1399.
15.
MiyaraFAubriotFXGlissantA. Multiparameter analysis of human oocytes at metaphase II stage after IVF failure in non-male infertility. Hum Reprod. 2003;18(7):1494–1503.
16.
YuYYanJLiuZC. Optimal timing of oocyte maturation and its relationship with the spindle assembly and developmental competence of in vitro matured human oocytes. Fertil Steril. 2011;96(1):73–8 e1.
17.
HeindryckxBDe GheselleSLiermanSGerrisJDe SutterP. Efficiency of polarized microscopy as a predictive tool for human oocyte quality. Hum Reprod. 2011;26(3):535–544.
18.
KorkmazCCinarOAkyolM. The relationship between meiotic spindle imaging and outcome of intracytoplasmic sperm injection: a retrospective study. Gynecol Endocrinol. 2011;27(10):737–741.
19.
PetersenCGOliveiraJBMauriAL. Relationship between visualization of meiotic spindle in human oocytes and ICSI outcomes: a meta-analysis. Reprod Biomed Online. 2009;18(2):235–243.
20.
TomariHHonjouKNagataYHoriuchiT. Relationship between meiotic spindle characteristics in human oocytes and the timing of the first zygotic cleavage after intracytoplasmic sperm injection. J Assist Reprod Genet. 2011;28(11):1099–1104.
21.
HyunCSChaJHSonWYYoonSHKimKALimJH. Optimal ICSI timing after the first polar body extrusion in in vitro matured human oocytes. Hum Reprod. 2007;22(7):1991–1995.
22.
DibLAAraujoMCGiorgenonRCFerrianiRANavarroPA. Apparently matured oocytes injected in telophase I have worse outcomes from assisted reproduction. Rev Bras Ginecol Obstet. 2012;34(5):203–208.
23.
MontagMKosterMvan der VenKvan der VenH. Gamete competence assessment by polarizing optics in assisted reproduction. Hum Reprod Update. 2011;17(5):654–666.
24.
BarcelosIDVieiraRCFerreiraEMMartinsWPFerrianiRANavarroPA. Comparative analysis of the spindle and chromosome configurations of in vitro-matured oocytes from patients with endometriosis and from control subjects: a pilot study. Fertil Steril. 2009;92(5):1749–1752.
25.
DibLAAraujoMCGiorgenonRCRomaoGSFerrianiRANavarroPA. Noninvasive imaging of the meiotic spindle of in vivo matured oocytes from infertile women with endometriosis. Reprod Sci. 2013;20(4):456–462.
26.
MoonJHHyunCSLeeSWSonWYYoonSHLimJH. Visualization of the metaphase II meiotic spindle in living human oocytes using the Polscope enables the prediction of embryonic developmental competence after ICSI. Hum Reprod. 2003;18(4):817–820.
27.
FangCTangMLiT. Visualization of meiotic spindle and subsequent embryonic development in in vitro and in vivo matured human oocytes. J Assist Reprod Genet. 2007;24(11):547–551.
28.
WangWHMengLHackettRJKeefeDL. Developmental ability of human oocytes with or without birefringent spindles imaged by Polscope before insemination. Hum Reprod. 2001;16(7):1464–1468.
29.
RienziLUbaldiFMartinezF. Relationship between meiotic spindle location with regard to the polar body position and oocyte developmental potential after ICSI. Hum Reprod. 2003;18(6):1289–1293.
30.
CohenYMalcovMSchwartzT. Spindle imaging: a new marker for optimal timing of ICSI?Hum Reprod. 2004;19(3):649–654.
31.
ShenYStalfTMehnertC. Light retardance by human oocyte spindle is positively related to pronuclear score after ICSI. Reprod Biomed Online. 2006;12(6):737–751.
32.
Rama RajuGAPrakashGJKrishnaKMMadanK. Meiotic spindle and zona pellucida characteristics as predictors of embryonic development: a preliminary study using PolScope imaging. Reprod Biomed Online. 2007;14(2):166–174.
33.
MadaschiCde Souza BonettiTCde Almeida Ferreira BragaDPPasqualottoFFIaconelliAJr, BorgesEJr. Spindle imaging: a marker for embryo development and implantation. Fertil Steril. 2008;90(1):194–198.
34.
Cristina MagliMCapotiARestaSStanghelliniIFerrarettiAPGianaroliL. Prolonged absence of meiotic spindles by birefringence imaging negatively affects normal fertilization and embryo development. Reprod Biomed Online. 2011;23(6):747–754.
35.
NavarroPALiuLFerrianiRAKeefeDL. Arsenite induces aberrations in meiosis that can be prevented by coadministration of N-acetylcysteine in mice. Fertil Steril. 2006;85suppl 1:1187–1194.
36.
KilaniSCookeSKanAChapmanM. Are there non-invasive markers in human oocytes that can predict pregnancy outcome?Reprod Biomed Online. 2009;18(5):674–680.
37.
AllworthAEAlbertiniDF. Meiotic maturation in cultured bovine oocytes is accompanied by remodeling of the cumulus cell cytoskeleton. Dev Biol. 1993;158(1):101–112.
38.
JuJCJiangSTsengJKParksJEYangX. Heat shock reduces developmental competence and alters spindle configuration of bovine oocytes. Theriogenology. 2005;64(8):1677–1689.
39.
FerreiraEMVirequeAAAdonaPRFerrianiRANavarroPA. Prematuration of bovine oocytes with butyrolactone I reversibly arrests meiosis without increasing meiotic abnormalities after in vitro maturation. Eur J Obstet Gynecol Reprod Biol. 2009;145(1):76–80.
40.
LiYFengHLCaoYJ. Confocal microscopic analysis of the spindle and chromosome configurations of human oocytes matured in vitro. Fertil Steril. 2006;85(4):827–832.
41.
OlmstedJBBorisyGG. Characterization of microtubule assembly in porcine brain extracts by viscometry. Biochemistry. 1973;12(21):4282–4289.
CookeSTylerJPDriscollGL. Meiotic spindle location and identification and its effect on embryonic cleavage plane and early development. Hum Reprod. 2003;18(11):2397–2405.
44.
WoodwardBJMontgomerySJHartshorneGMCampbellKHKennedyR. Spindle position assessment prior to ICSI does not benefit fertilization or early embryo quality. Reprod Biomed Online. 2008;16(2):232–238.
45.
TaylorTHChangCCElliottTColturatoLFKortHINagyZP. Effect of denuding on polar body position in in-vitro matured oocytes. Reprod Biomed Online. 2008;17(4):515–519.
