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Abstract

Background

Quantitative T₂ relaxation magnetic resonance imaging allows estimation of stroke onset time.

Aims

We aimed to examine the accuracy of quantitative T₁ and quantitative T₂ relaxation times alone and in combination to provide estimates of stroke onset time in a rat model of permanent focal cerebral ischemia and map the spatial distribution of elevated quantitative T₁ and quantitative T₂ to assess tissue status.

Methods

Permanent middle cerebral artery occlusion was induced in Wistar rats. Animals were scanned at 9.4T for quantitative T₁, quantitative T₂, and Trace of Diffusion Tensor (D_av) up to 4 h post-middle cerebral artery occlusion. Time courses of differentials of quantitative T₁ and quantitative T₂ in ischemic and non-ischemic contralateral brain tissue (ΔT₁, ΔT₂) and volumes of tissue with elevated T₁ and T₂ relaxation times (f₁, f₂) were determined. TTC staining was used to highlight permanent ischemic damage.

Results

ΔT₁, ΔT₂, f₁, f₂, and the volume of tissue with both elevated quantitative T₁ and quantitative T₂ (V^Overlap) increased with time post-middle cerebral artery occlusion allowing stroke onset time to be estimated. V^Overlap provided the most accurate estimate with an uncertainty of ±25 min. At all times-points regions with elevated relaxation times were smaller than areas with D_av defined ischemia.

Conclusions

Stroke onset time can be determined by quantitative T₁ and quantitative T₂ relaxation times and tissue volumes. Combining quantitative T₁ and quantitative T₂ provides the most accurate estimate and potentially identifies irreversibly damaged brain tissue.

Keywords

Ischemic stroke magnetic resonance imaging onset time

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Stroke onset time estimation from multispectral quantitative magnetic resonance imaging in a rat model of focal permanent cerebral ischemia

Abstract

Background

Aims

Methods

Results

Conclusions

Keywords

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References

Supplementary Material