Anti-ΔIK17 monoclonal antibody was produced by fusing SP2/0/Ag14 myeloma with spleen cells of BALB/c mice immunized with normal human thymocytes. AΔIK17 antibody recognizes a 44kD cell surface protein detected on human lymphocytes. ΔIK17 is expressed on human thymocytes, CD4+ and CD8+ T cell subsets, B, NK cells, as well as on activated cells. The antigen is detected on cells during the early, indermediate and late stages of lymphocyte maturation. In addition, the expression of the antigen is correlated with ontogenesis. A T+ ΔIK17+ subpopulation responded poorly to TPA stimulation and provided a better helper signal for PWM-induced IgM synthesis than T+ ΔIK17- cells. In addition, different levels of ΔIK17 expression were detected in several hematological diseases tested.
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