Transcriptional Activation of the Human Immunodeficiency Virus Long Terminal Repeat Sequences by Retinoic Acid in Human Epithelial and Fibroblast Tumor Cell Lines
Restricted accessResearch articleFirst published online July, 1996
Transcriptional Activation of the Human Immunodeficiency Virus Long Terminal Repeat Sequences by Retinoic Acid in Human Epithelial and Fibroblast Tumor Cell Lines
We employed a recombinant plasmid, pBHIV1, carrying the long terminal repeat (LTR) sequences of HIV-1 linked to the reporter chloramphenicol acetyl transferase (CAT) gene and to the aminoglycoside phosphotransferase (aph) gene as a selectable marker. We introduced pBHIV1 into human epithelial and fibroblast tumor cell lines (HeLa and MRCSV40TGR), and obtained stable geneticin-resistant HLHIV1-A and SVTGHIV1-A cells, respectively. The response to the retinoic acid was studied on the LTR regulated CAT activity in both cell lines. It was found that retinoic acid at a concentration of 1×10−5 effects a 3.2 - fold increase in CAT expression compared to HIV LTR in HLHIV1-A, but requires a concentration of 5×10−5 M to enhance this expression 4.6-fold in SVTGHIV1-A cells. These data show that retinoic acid may play a critical role in HIV-1 expression in human epithelial and fibroblast cell lines.
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