Restricted accessResearch articleFirst published online 1996-7
Transcriptional Activation of the Human Immunodeficiency Virus Long Terminal Repeat Sequences by Retinoic Acid in Human Epithelial and Fibroblast Tumor Cell Lines
We employed a recombinant plasmid, pBHIV1, carrying the long terminal repeat (LTR) sequences of HIV-1 linked to the reporter chloramphenicol acetyl transferase (CAT) gene and to the aminoglycoside phosphotransferase (aph) gene as a selectable marker. We introduced pBHIV1 into human epithelial and fibroblast tumor cell lines (HeLa and MRCSV40TGR), and obtained stable geneticin-resistant HLHIV1-A and SVTGHIV1-A cells, respectively. The response to the retinoic acid was studied on the LTR regulated CAT activity in both cell lines. It was found that retinoic acid at a concentration of 1×10−5 effects a 3.2 - fold increase in CAT expression compared to HIV LTR in HLHIV1-A, but requires a concentration of 5×10−5 M to enhance this expression 4.6-fold in SVTGHIV1-A cells. These data show that retinoic acid may play a critical role in HIV-1 expression in human epithelial and fibroblast cell lines.
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