Immunological Properties of a Radioiodinated Monoclonal Antibody and Its F(ab') 2μ Fragments Directed Against the Polymorphic Epithelial Mucin of Human Breast Cancer
Restricted accessResearch articleFirst published online October, 1992
Immunological Properties of a Radioiodinated Monoclonal Antibody and Its F(ab') 2μ Fragments Directed Against the Polymorphic Epithelial Mucin of Human Breast Cancer
The purification of the IgM monoclonal antibody 436 against a breast tumor antigen from mouse ascitic fluid is reported. The purified immunoglobulin was radioiodinated and the resulting product assessed for its binding capacity and binding specificity. Purified IgM-436 served for F(ab') 2μpreparation which was tested for its antigen binding capacity. Radioiodinated IgM-436 and its F(ab')2μ retained their immunological activity which was never lower than those of the corresponding cold products.
GoldenbergD.M., KimE.E., BennetS.J.Carcinoembryonic antigen radioimmunodetection in the evaluation of colorectal cancer and in the detection of occult neoplasms.Gastroenterology1983; 84: 524–32.
2.
GoldenbergD.M., KimE.E., DelandF.H.Human chorionic gonadotropin radioantibodies in the radioimmunodetection of cancer and for disclosure of occult metastases.Proc Natl Acad Sci (Wash)1981; 78: 7754–8.
3.
GoldenbergD.M., KimE.E., DelandF.H.Clinical studies of radioimmunodetection of tumors containing alpha-fetoprotein.Cancer1980; 45: 2500–4.
4.
SiccardiA.G., BuraggiG.L., CallegaroL.Immunoscintigraphy of adenocarcinomas by means of radiolabeled F(ab')2 fragments of an anti-carcinoembryonic antigen monoclonal antibody: a multicenter study.Cancer Res1989; 49: 3095–103.
5.
GoldenbergD.M., BlumenthalR.D., SharkeyR.M.Biological and clinical perspectives of cancer imaging and therapy with radiolabeled antibodies.Sem Cancer Biol1990; 1: 217–25.
6.
TjandraJ., RusselI.S., CollinsJ.P.Immunolymo-hoscintigraphy for the detection of lymph node metastases from breast cancer.Cancer Res1989; 49: 1600–8.
7.
EpenetosA.A., MatherS., GranowskaM.Targeting of I123-labelled tumor-associated monoclonal antibodies to ovarian breast and gastrointestinal tumors.Lancet1982; 2: 999–1004.
8.
BallouB., ReilandJ., LevineG., KnowelesB., HakalaT.R.Tumor location using F(ab')2μ from a monoclonal IgM antibody: pharmacokinetics.J Nucl Med1985; 26: 283–92.
9.
NutiM., TurchiV., MasciA.M.Monoclonal antibody (MoAb) 436 recognizing a breast carcinoma associated mucin: spectrum of reactivity. 1st International workshop on carcinoma-associated mucins 1990; San Francisco, California, USA.
10.
TurchiV., MasciA.M., RughettiA.Characterization of monoclonal antibody (MoAb) 436 against a tumor associated epitope of a breast mucin.International association for breast cancer research1991; St. Vincent, Aosta. Italy.
11.
NutiM., TurchiV., MasciA.M.Characterization of monoclonal antibody 436 recognizing the arg-pro-ala-pro sequence of the polymorphic epithelial mucin (PEM) protein core in breast carcinoma cells. Submitted for publication.
BurchellJ., Taylor-PapadimitriouJ., BoshellM., GendlerS., DuhigT.A short sequence, within the aminoacids tandem repeat of a cancer associated mucin, contains immunodominant epitopes.Int J Cancer1989; 44: 691–6.
14.
BealeD., Van DortT.A comparison of the proteolytic fragmentation of immunoglobulin M from several different mammalian species.Comp Biochem Physiol1982; 71B: 475–83.
15.
MatthewW.D., ReichardtF.Development and application of an efficient procedure for converting mouse IgM into small, active fragments.J Immunol Meth1982; 50: 239–53.
16.
ParhamP.Preparation and purification of active fragments from mouse monoclonal antibodies. In: WeirD.M., HerzembergL.A., BlackwellC., Herzemberg LeonoreA., eds. Immunochemistry.Oxford: Blackwell Scientific Publications, 1986; 14.18–14.19.
