Iron bioavailability was determined by the extrinsic label method in seven diets consumed by low, middle, and high socio-economic strata of the Venezuelan population. The results were compared with physiologic iron requirements and the prevalence of iron deficiency according to age and sex of others in the same strata. The iron metabolic balance obtained by comparing the iron absorption from diets with the physiologic iron requirement was negative in subjects consuming the low-bioavailability diets, being more marked in children below four years of age, and in adolescents and adult females. These groups also showed the highest prevalence of iron deficiency with and without anaemia. The metabolic iron balance in subjects consuming high iron-bioavailability diets was less negative among vulnerable groups consuming low-bioavailability diets and positive in the age and sex groups less vulnerable to iron deficiency. This information was used to identify the principal categories of dietary iron bioavailability in various regions of the world and their effect on the prevalence of iron deficiency.
References
1.
CookJ., LayrisseM., Martínez-TorresC., WalkerR., MonsenE., FinchC.A.Food iron absorption measured by an extrinsic tag. J Clin Invest1972; 51: 805–15.
2.
Björn-RasmussenE., HallbergL., WalkerR.B.Food iron absorption in man. I. Isotopic exchange between food iron and inorganic iron salt added to food: studies on maize, wheat, and eggs. Am J Clin Nutr1972; 25: 317–23.
3.
LayrisseM., Martinez-TorresC.Model for measuring dietary absorption of heme iron: test with a complete meal. Am J Clin Nutr1972; 25: 401–11.
4.
LayrisseM., Martinez-TorresC., GonzalezM.Measurement of the total daily dietary absorption by the extrinsic tag mode. Am J Clin Nutr1974; 27: 152–62.
5.
Björn-RasmussenE., HallbergL., IsakssonB., ArvidssonB.Food iron absorption in man: application of the two-pool extrinsic tag method to measure heme and nonheme iron absorption from the whole diet. J Clin Invest1974; 53: 247–55.
6.
CookJ.D., MonsenE.R.Food iron absorption. I. Use of a semisynthetic diet to study absorption of nonheme iron. Am] Clin Nutr1975; 28: 1289–95.
7.
CookJ.D., MonsenE.R.Food iron absorption in human subjects. III. Comparison of the effect of animal proteins on nonheme iron absorption. Am J Clin Nutr1975; 28: 859–67.
8.
Than BatuA., ThanT., ToeT.Iron absorption from south Asian rice based meals. Am J Clin Nutr1976; 29: 129225.
9.
ThauT., ToeT., ThwinM.G., Than-BatuA.Isotopic exchange in 59Fe labelled rice based meals. J Lab Clin Med1977; 90: 231–37.
10.
HallbergL., Björn-RasmussenE., RossanderL., SuwanikR.Iron absorption from southeast Asia diets. ll. Role of various factors that might explain low absorption. Am J Clin Nutr1977; 30: 539–48.
11.
MonsenE.R., CookJ.D.Food iron absorption in human subjects. V. Effects of the major dietary constituents of a semisynthetic meal. Am J Clin Nutr1977; 30: 804–08.
12.
RossanderL., HallbergL., Björn-RasmussenE.Absorption of iron from breakfast meals. Am J Clin Nutr1979; 32: 2484–89.
13.
HallbergL., RossanderL.Absorption of iron from western-type of lunch and dinner meals. Am J Clin Nutr1982; 35: 502–09.
14.
HallbergL., Björn-RasmussenE., RossanderL., SuwanikR., PleehachindaR., MaluleeT.Iron absorption from some Asian meals containing contamination iron. Am J Clin Nutr1983; 37: 272–77.
15.
AcostaA., AmarM., Cornbluth-SzarfarcS.Iron absorption from typical Latin American diets. Am J Clin Nutr1984; 39: 953–62.
16.
FossiM., Mendez-CastellanoH., JaffeW.Perfil hematológico y absorción del hierro de las dicta que consumer estratos socio-económicos bajos de la población de dos estados de Venezuela. Arch Lat Am Nutr1987; 37: 23–25.
Mendez-CastellanoH., López-BlancoM.F., Landaeta-JiménezM., FossiM., BoschV., Mijares-GilA.Visión del “Proyecto Venezuela” sobre evaluación de la situación nutricional.Caracas, Venezuela: Fundacredesa, 1985.
19.
Méndez-CastellanoH., MendezM.C.Estratificación social y humane: método de Graffar modificado. Arch Venez Puer Pediatr1986; 49: 93–104.
20.
LayrisseM.Deficiencia de hierro en América Latina. Bol Soc Brasilera Hematol1988; 10: 92–97.
21.
LayrisseM., Martinez-TorresC., TaylorP.Requerimientos de nutrientes que participan en la eritropoyesis. Taller de guias nutricionales pare America Latina. Arch Lat Am Nutr1988; 38: 250–74.
22.
LayrisseM.Food iron availability. Proceedings of the 14th International Congress of Nutrition, Seoul, Korea, 1990
23.
GraffarM.Une méthode de classification social échantillons depopulation. Courrier1956; 6: 445–59.
24.
CrosbyW.H., MunnJ.L., FurthF.W.Standardizing a method for clinical hemoglobinometry. US Armed Forces Med J1954; 5: 693–703.
25.
International Committee for Standardization in Hematology.Recommendation for measurement of serum iron in human blood. Br J Haematol1978; 38: 291–94.
26.
International Committee for Standardization in Hematology.The measurement of total and saturated iron-binding capacity in serum. Br J Haematol1978; 38: 281–90.
27.
MilesL.E.M., LipschitzDA., BieberCP., CookJ.D.Measurement of serum ferritin by a 2-site immunoradiometric assay. Ann Biochem1974; 61: 209–24.
28.
FlowersC.A., KuizonM., BeardSL., SkiknerBS., CovellA.M., CookJ.D.A serum ferritin assay for prevalence studies of iron deficiency. Am J Hematol1986; 23: 141–51.
29.
LayrisseM., CookJ.D., Martinez-TorresC., RocheM., KuhnI.N., FinchC.A.Food iron absorption: a comparison of vegetable and animal foods. Blood1969; 33: 430–43.
30.
DernJ.R., HartW.L.Studies with doubly labelled iron. I. Simultaneous liquid scintillation counting isotopes of Fe55 and Fe59 as ferrous Perchlorate. J Lab Clin Med1961; 57: 322–30.
31.
DernJ.R., HartW.L.Studies with doubly labelled iron. II. Separation of iron from blood samples and preparation of ferrous perchlorate for liquid scintillation counting. J Lab Clin Med1961; 57: 460–67.
32.
NadlerS.B., HidalgoJ.U., BlochT.The Tulane table of blood volume in normal men. Surgery1962; 51: 224–32.
TaylorP., Martinez-TorresC., LeetsI., RamirezJ., Garcia-CasalM.N., LayrisseM.Relationships among iron absorption, percent saturation of plasma transferrin and serum ferritin concentration. JNutr 198K; 118: 1110–15.
35.
SchrickerB.R., MillerD.D., StaufferJ.R.Measurement and content of nonheme and total iron in muscle. J Food Sci1982; 47: 740–43.
36.
HaugW., LantzchH.J.Sensitive method for the rapid determination of phytate in cereals and cereal products. J Sci Food Agric1982; 34: 1423–26.
37.
PriceML., ButlerL.Rapid visual estimation and spectrophotometric determination of tannin content of sorghum grain. J Agric Food Chem1977; 25: 1268–73.
38.
TaylorP., Mendez-CastellanoH., López-BiancoM.Daily physiological iron requirements in children. J Am Diet Assoc1988; 88: 454–58.
39.
Center for Food Safety and Applied Nutrition.Assessment of the iron nutritional status of the US population based on data collected in the second national health and nutrition examination survey, 1976–1980.Washington, DC: Government Printing Office, 1984.
DislerPB., LynchS.R., CharltonR.W., BothwellT.The effect of tea on iron absorption. Gut1975; 16: 193–20.
42.
Martínez-TorresC., RomanoEL., RenziM., LayrisseM.Fe(III)-EDTA complex as iron fortification: further studies. Am J Clin Nutr1979; 32: 809–16.
43.
MorckT.A., LynchS.R., SkikneB.S., CookJ.D.Iron availability from infant food supplements. Am J Clin Nutr1981; 34: 2630–34.
44.
MorckT.A., LynchS.R., CookJ.D.Inhibition of food iron absorption by coffee. Am J Clin Nutr1983; 37: 416–20.
45.
LynchS.R., BeardJ.L., Dassen KoS.A., CookJ.D.Iron absorption from legumes in humans. Am J Clin Nutr1984; 40: 42–47
46.
HallbergL.Bioavailable nutrient density: a new concept applied in the interpretation of food iron absorption data. Am J Clin Nutr1981; 34: 2242–47.
47.
HallbergL., BruneM., RossanderL.Iron absorption in man: ascorbic acid and dose-dependent inhibition by phytate. Am J Clin Nutr1989; 49: 140–44.
48.
GillodyM., BothwellT.H., TorranceJD. The effect of organic acids, phytates and polyphenols on the absorption of iron from vegetables. Br J Nutr1983; 49: 33142.
49.
GillodyM., BothwellT.H., CharltonR.W.Factors affecting the absorption of iron from cereals. Br J Nutr1984; 51: 37–46.
50.
Requirements of vitamin A, iron, folate, and vitamin B12.Joint FAD/WHO expert consultation.Rome: FAO, 1988.
51.
SigulemD.M., TudiscoE.S., GoldenbergP., AthaideM.M., WaismanE.Anemias e deficiencias de ferro em crianças do municipio de São Paulo. Rev Saúde Publica1978; 12: 168–97.
